Lanostanes from Phellinus igniarius and Their iNOS Inhibitory Activities

被引：66

作者：

Wang, Guei-Jane ^{[1
]}

Tsai, Tung-Hu ^{[2
]}

Chang, Tun-Tschu ^{[3
]}

Chou, Cheng-Jen ^{[1
]}

Lin, Lie-Chwen ^{[1
,2
,4
]}

机构：

[1] Natl Res Inst Chinese Med, Taipei 112, Taiwan

[2] Natl Yang Ming Univ, Inst Tradit Med, Taipei 112, Taiwan

[3] Taiwan Forestry Res Inst, Div Forest Protect, Taipei, Taiwan

[4] China Med Univ, Grad Inst Integrated Med, Taichung, Taiwan

来源：

PLANTA MEDICA | 2009年 / 75卷 / 15期

关键词：

Phellinus igniarius; Hymenochaetaceae; triterpenoids; igniarens; inflammation; macrophages; nitric oxide; NITRIC-OXIDE SYNTHASE; TRITERPENOIDS; DERIVATIVES; ANTIOXIDANT; CELLS;

D O I：

10.1055/s-0029-1185813

中图分类号：

Q94 [植物学];

学科分类号：

071001 [植物学];

摘要：

Four new lanostanol-type triterpenoids, igniarens A - D (1-4), were isolated from the fruit body of Phellinus igniarius together with two known triterpenoids, and two known ergostanes. These four new compounds were identified by spectroscopic analysis as 22R-hydroxy-24-methylene-29-norlanost-7, 9(11)-dien-3-one (1), 3 alpha,22R-dihydroxy-24-methylene-29-norlanost-7, 9(11)-diene (2), 3 alpha,22R-dihydroxy-24-methylene-29-norlanost-8-ene (3), and 3 alpha,22R-dihydroxy-24-methylenelanost-8-ene (4). Their effects on NO production in lipopolysaccharide (LPS)-activated macrophages were assessed. Compounds 1-8 inhibited NO production in activated RAW 264.7 cells to various degrees. The most potent compound 5 alpha,8 alpha-epidioxy-22E-ergosta-6,22-dien-3 beta-ol (7) significantly inhibited LPS-induced NO production in a concentration-dependent manner without affecting the cellular viability, with an IC50 of 37.57 +/- 1.38 mu M.

引用

页码：1602 / 1607

页数：6

共 20 条

[1]

4-ALPHA,14-ALPHA-DIMETHYL-5-ALPHA-ERGOSTA-7,9(11),24(28)-TRIEN-3-BETA-OL FROM PHASEOLUS-VULGARIS AND GYNOSTEMMA-PENTAPHYLLUM [J].