On-Chip Immunoassay for Determination of Urinary Albumin

被引：26

作者：

Laiwattanapaisal, Wanida ^{[1
]}

Songjaroen, Temsiri ^{[2
]}

Maturos, Thitima ^{[3
]}

Lomas, Tanom ^{[3
]}

Sappat, Assawapong ^{[3
]}

Tuantranont, Adisorn ^{[3
]}

机构：

[1] Chulalongkorn Univ, Ctr Excellence Om Nano Med Technol Project Dev, Dept Clin Chem, Fac Allied Hlth Sci, Bangkok 10330, Thailand

[2] Chulalongkorn Univ, Grad Programme Clin Biochem & Mol Med, Fac Allied Hlth Sci, Bangkok 10330, Thailand

[3] Natl Elect & Comp Technol Ctr, Nanoelect & MEMS Lab, Pathum Thani 12120, Thailand

来源：

SENSORS | 2009年 / 9卷 / 12期

关键词：

microalbuminuria; microfluidic system; on-chip immunoassay; poly(dimethylsiloxane); LATEX IMMUNOAGGLUTINATION ASSAY; POLY(DIMETHYLSILOXANE); IMMUNOSENSOR; VALIDATION; DIAGNOSIS; DEVICE; SERUM;

D O I：

10.3390/s91210066

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An immunoassay performed on a portable microfluidic device was evaluated for the determination of urinary albumin. An increase in absorbance at 500 nm resulting from immunoagglutination was monitored directly on the poly(dimethylsiloxane) (PDMS) microchip using a portable miniature fibre-optic spectrometer. A calibration curve was linear up to 10 mg L-1 (r(2) = 0.993), with a detection limit of 0.81 mg L-1 (S/N = 3). The proposed system showed good precision, with relative standard deviations (RSDs) of 5.1%, when evaluated with 10 mg L-1 albumin (n = 10). Determination of urinary albumin with the proposed system gave results highly similar to those determined by the conventional spectrophotometric method using immunoturbidimetric detection (r(2) = 0.995; n = 15).

引用

页码：10066 / 10079

页数：14

共 33 条

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