Melatonin suppresses the induction of AP-1 transcription factor components in the pars tuberalis of the pituitary

被引:38
作者
Ross, AW
Barrett, P
Mercer, JG
Morgan, PJ
机构
[1] Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, Greenburn Road
基金
英国生物技术与生命科学研究理事会;
关键词
melatonin; pars tuberalis; gene expression; c-fos; AP-1; pituitary;
D O I
10.1016/0303-7207(96)03897-X
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
In ovine pars tuberalis cells which express high affinity Mel la melatonin receptors, the ability of melatonin to directly stimulate or inhibit AP-1 transcription factor gene expression was studied. Effects of melatonin upon mRNA expression by forskolin, serum and IGF-1 were also investigated. Northern analysis showed melatonin had no direct stimulatory nor inhibitory effect upon transcription or translation. Melatonin was able to significantly inhibit forskolin-stimulated induction of c-fos and jun B mRNA whilst forskolin had no effect upon c-jun or jun D. Induction of c-Fos translation by forskolin was also inhibited by melatonin. Serum induced c-fos and c-jun, but melatonin was unable to affect these changes. Similarly IGF-1 stimulated c-fos and melatonin had no effect upon this induction. From these results it can be concluded that melatonin has no independent effects on expression of the AP-1 genes, rather its primary function is to inhibit cell activities through cyclic AMP-dependent routes of gene activation.
引用
收藏
页码:71 / 80
页数:10
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