Mesenchymal stem cells from cryopreserved human umbilical cord blood

被引:128
作者
Lee, MW
Choi, J
Yang, MS
Moon, YJ
Park, JS
Kim, HC
Kim, YJ [1 ]
机构
[1] LifeCord Inc, Biomed Res Inst, Suwon, South Korea
[2] Ajou Univ, Sch Med, Dept Hematol Oncol, Suwon, South Korea
关键词
cryopreservation; umbilical cord blood; mesenchymal stem cells;
D O I
10.1016/j.bbrc.2004.04.206
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Umbilical cord blood (UCB) is well known to be a rich source of hematopoietic stem cells with practical and ethical advantages, but the presence of mesenchymal stem cells (MSCs) in UCB has been disputed and it remains to be validated. In this study, we examined the ability of cryopreserved UCB harvests to produce cells with characteristics of MSCs. We were able to obtain homogeneous plastic adherent cells from the mononuclear cell fractions of cryopreserved UCB using our culture conditions. These adherent cell populations exhibited fibroblast-like morphology and typical mesenchymal-like immunophenotypes (CD73(+), CD105(+), and CD 166(+), etc.). These cells presented the self-renewal capacity and the mesenchymal cell-lineage potential to form bone, fat, and cartilage. Moreover, they expressed mRNAs of multi-lineage genes including SDF-1, NeuroD, and VEGF-R1, suggesting that the obtained cells had the multi-differentiation capacity as bone marrow-derived MSCs. These results indicate that cryopreserved human UCB fractions can be used as an alternative source of MSCs for experimental and therapeutic applications. (C) 2004 Elsevier Inc. All rights reserved.
引用
收藏
页码:273 / 278
页数:6
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