Molecular cloning and expression analysis of carp (Cyprinus carpio) interleukin-1β, high affinity immunoglobulin E Fc receptor γ subunit and serum amyloid A

被引:173
作者
Fujiki, K [1 ]
Shin, DH [1 ]
Nakao, M [1 ]
Yano, T [1 ]
机构
[1] Kyushu Univ, Fac Agr, Marine Biochem Lab, Fukuoka 8128581, Japan
关键词
carp; IL-1; beta; Fc epsilon RI gamma; serum amyloid A; suppression subtractive hybridisation; inflammation;
D O I
10.1006/fsim.1999.0253
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
Suppression subtractive hybridisation (SSH) is a powerful means to identify genes of cytokines and other genes that express small amount of mRNA. In this study, cDNA of normal fish (carp) head kidney cells (HKC) was subtracted from pooled cDNA of HKC and peritoneal cell (PC) obtained from fish which had been injected with sodium alginate (SA) and scleroglucan (SG) 3-48 h earlier. This subtraction produced 248 clones of cDNA fragments. After sequencing some of the fragments of interest were used as probes, and yielded full-length cDNAs homologous to mammalian interleukin-1 beta (IL-1 beta), the gamma subunit of high affinity Fc receptor for IgE (Fc epsilon RI gamma) and serum amyloid A (SAA); these were cloned and sequenced. Carp IL-1 beta shows 21.8-24.7% amino acid identities to mammalian mature IL-1 beta, and lacks a signal sequence, which is consistent with mammalian IL-1 beta. Carp Fc epsilon RI gamma, which was the first cloned non-mammalian Fc receptor subunit, shows 39.3-40.4% amino acid identities to mammalian Fc epsilon RII gamma, and contains the immunoreceptor tyrosin-based activation motif characteristic of the signal transduction subunit of antigen- and Fc-receptors. Carp SAA is most similar to mammalian acute phase responsive type SAA with 53.0-55.3% amino acid identities. Both SA-elicited and SC-elicited PC expressed higher amounts of IL-1 beta and SAA mRNA compared to saline-injected fish HKC and PC, indicating that these proteins are associated with inflammatory responses, similar to mammalian homologues. Fc epsilon RI gamma was constitutively expressed in leucocytes and not immunopotentiator-responsive, but this indicates that Fc receptor including Fc epsilon RI gamma subunit is likely functional in the carp immune system. (C) 2000 Academic Press.
引用
收藏
页码:229 / 242
页数:14
相关论文
共 42 条
  • [1] ANDREWS AE, 1991, IMMUNOLOGY, V74, P453
  • [2] ARINAGA S, 1992, INT J IMMUNOPHARMACO, V14, P43
  • [3] NUCLEOTIDE-SEQUENCE OF HUMAN MONOCYTE INTERLEUKIN-1 PRECURSOR CDNA
    AURON, PE
    WEBB, AC
    ROSENWASSER, LJ
    MUCCI, SF
    RICH, A
    WOLFF, SM
    DINARELLO, CA
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (24): : 7907 - 7911
  • [4] SERUM AMYLOID-A IS A CHEMOATTRACTANT - INDUCTION OF MIGRATION, ADHESION, AND TISSUE INFILTRATION OF MONOCYTES AND POLYMORPHONUCLEAR LEUKOCYTES
    BADOLATO, R
    WANG, JM
    MURPHY, WJ
    LLOYD, AR
    MICHIEL, DF
    BAUSSERMAN, LL
    KELVIN, DJ
    OPPENHEIM, JJ
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1994, 180 (01) : 203 - 209
  • [5] BAGGIOLINI M, 1994, ADV IMMUNOL, V55, P97
  • [6] Dinarello C A, 1998, Int Rev Immunol, V16, P457, DOI 10.3109/08830189809043005
  • [7] FISH MAJOR HISTOCOMPATIBILITY COMPLEX GENES - AN EXPANSION
    DIXON, B
    VANERP, SHM
    RODRIGUES, PNS
    EGBERTS, E
    STET, RJM
    [J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 1995, 19 (02) : 109 - 133
  • [8] NUCLEOTIDE-SEQUENCE OF OVINE INTERLEUKIN-1-BETA
    FISKERSTRAND, C
    SARGAN, D
    [J]. NUCLEIC ACIDS RESEARCH, 1990, 18 (23) : 7165 - 7165
  • [9] THE EFFECT OF LENTINAN ON PRODUCTION OF INTERLEUKIN-1 BY HUMAN-MONOCYTES
    FRUEHAUF, JP
    BONNARD, GD
    HERBERMAN, RB
    [J]. IMMUNOPHARMACOLOGY, 1982, 5 (01): : 65 - 74
  • [10] Molecular cloning of carp (Cyprinus carpio) CC chemokine, CXC chemokine receptors, allograft inflammatory factor-1, and natural killer cell enhancing factor by use of suppression subtractive hybridization
    Fujiki, K
    Shin, DH
    Nakao, M
    Yano, T
    [J]. IMMUNOGENETICS, 1999, 49 (10) : 909 - 914