Automated synthesis of double dye-labeled oligonucleotides using tetramethylrhodamine (TAMRA) solid supports.

被引：25

作者：

Mullah, B

Andrus, A

机构：

[1] Applied Biosystems Division, Perkin Elmer Corporation, Foster City, CA 94404

来源：

TETRAHEDRON LETTERS | 1997年 / 38卷 / 33期

关键词：

D O I：

10.1016/S0040-4039(97)01286-0

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

TAMRA fluorescent dye-labeled non-nucleosidic synthesis supports have been derivatized for automated synthesis of 3'dye-labeled oligonucleotides.(1) Synthesis with 5' fluorescent dye phosphoramidites leads to 5' and 3'double labeled oligonucleotides, useful in Taqman assay and FRET experiments. Cleavage and deprotection of dye-labeled dye-labeled oligonucleotides are carried out with tert-butylamine:methnnol:water (1:1:2) without any degradation and/or modification of dyes and nucleobases. (C) 1997 Elsevier Science Ltd.

引用

页码：5751 / 5754

页数：4

共 16 条

[1] OXALYL-CPG - A LABILE SUPPORT FOR SYNTHESIS OF SENSITIVE OLIGONUCLEOTIDE DERIVATIVES [J].