Glutathione and gamma-glutamyl transferases are involved in the formation of cadmium-glutathione complex

被引：19

作者：

Braga Adamis, Paula Daniela ^{[1
]}

Mannarino, Sergio Cantu ^{[1
]}

Araujo Eleutherio, Elis Cristina ^{[1
]}

机构：

[1] Univ Fed Rio de Janeiro, IQ, Dept Bioquim, BR-21941909 Rio de Janeiro, Brazil

来源：

FEBS LETTERS | 2009年 / 583卷 / 09期

关键词：

Cadmium; Gamma-glutamyl transferase; Glutathione transferase; Glutathione; Saccharomyces cerevisiae; YEAST SACCHAROMYCES-CEREVISIAE; TRANSPEPTIDASE; TRANSPORT; CELLS; METABOLISM; PATHWAY;

D O I：

10.1016/j.febslet.2009.03.066

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In a wild-type strain of Saccharomyces cerevisiae, cadmium induces the activities of both gamma-glutamyl transferase (gamma-GT) and glutathione transferase 2 (Gtt2). However, Gtt2 activity did not increase under gamma-GT or Ycf1 deficiencies, suggesting that the accumulation of glutathione- cadmium in the cytosol inhibits Gtt2. On the other hand, the balance between the cytoplasmic and vacuolar level of glutathione seems to regulate gamma-GT activity, since this enzyme was not activated in a gtt2 strain. Taken together, these results suggest that gamma-GT and Gtt2 work together to remove cadmium from the cytoplasm, a crucial mechanism for metal detoxification that is dependent on glutathione. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：1489 / 1492

页数：4

共 21 条

[1] Vacuolar compartmentation of the cadmium-glutathione complex protects Saccharomyces cerevisiae from mutagenesis [J].

Adamis, Paula D. B. ;

Panek, Anita D. ;

Eleutherio, Elis C. A. .

TOXICOLOGY LETTERS, 2007, 173 (01) :1-7

[2]

ADAMIS PD, 2008, BIOMETALS

[3] The role of glutathione transferases in cadmium stress [J].

Adamis, PDB ;

Gomes, DS ;

Pinto, MLCC ;

Panek, AD ;

Eleutherio, ECA .

TOXICOLOGY LETTERS, 2004, 154 (1-2) :81-88

[4]

Anderson ME, 1998, CHEM-BIOL INTERACT, V112, P1

[5] Metabolism and functions of glutathione in brain [J].

Dringen, R .

PROGRESS IN NEUROBIOLOGY, 2000, 62 (06) :649-671

[6] Sulfur sparing in the yeast proteome in response to sulfur demand [J].

Fauchon, M ;

Lagniel, G ;

Aude, JC ;

Lombardia, L ;

Soularue, P ;

Petat, C ;

Marguerie, G ;

Sentenac, A ;

Werner, M ;

Labarre, J .

MOLECULAR CELL, 2002, 9 (04) :713-723

[7] Apoptosis as a mechanism for removal of mutated cells of Saccharomyces cerevisiae:: The role of Grx2 under cadmium exposure [J].

Gomes, Debora Silva ;

Pereira, Marcos Dias ;

Panek, Anita Dolly ;

Andrade, Leonardo Rodrigues ;

Araujo Eleutherio, Elis Cristina .

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2008, 1780 (02) :160-166

[8] Regulation of cadmium uptake by Saccharomyces cerevisiae [J].

Gomes, DS ;

Fragoso, LC ;

Riger, CJ ;

Panek, AD ;

Eleutherio, ECA .

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2002, 1573 (01) :21-25

[9]

*IARC, 1993, IARC CANC MON EV CAR

[10] Cadmium is a mutagen that acts by inhibiting mismatch repair [J].

Jin, YH ;

Clark, AB ;

Slebos, RJC ;

Al-Refai, H ;

Taylor, JA ;

Kunkel, TA ;

Resnick, MA ;

Gordenin, DA .

NATURE GENETICS, 2003, 34 (03) :326-329

← 1 2 3 →