Determination of MDMA, MDEA and MDA in urine by high performance liquid chromatography with fluorescence detection

被引：32

作者：

da Costa, JL ^{[1
]}

Chasin, AAD

机构：

[1] Univ Sao Paulo, Coll Pharmaceut Sci Toxicol, Instrumental Anal Lab, Criminalist Inst, Av Prof Lineu Prestes,580 B13B Toxicol, BR-05508900 Sao Paulo, Brazil

[2] Coll Oswaldo Cruz, Forens Toxicol Lab, Legal Med Inst, Sao Paulo, Brazil

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2004年 / 811卷 / 01期

关键词：

drug of abuse; MDMA; MDEA;

D O I：

10.1016/j.jchromb.2004.03.076

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This paper describes the development and validation of analytical methodology for the determination of the use of MDMA, MDEA and MDA in urine. After a simple liquid extraction, the analyses were carried out on a high performance liquid chromatography (HPLC) in an octadecyl column, with fluorescence detection. The mobile phase using a sodium dodecyl sulfate ion-pairing reagent allows good separation and efficiency. The method showed good linearity and precision. Recovery was between 85 and 102% and detection limits were 10, 15 and 20 ng/ml for MDA, MDMA and MDEA, respectively. No interfering substances were detected with fluorescence detection. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：41 / 45

页数：5

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