High-performance liquid chromatographic determination of phenol, 4-nitrophenol, beta-naphthol and a number of their glucuronide and sulphate conjugates in organ perfusate

被引：57

作者：

Thompson, MJ ^{[1
]}

Ballinger, LN ^{[1
]}

Cross, SE ^{[1
]}

Roberts, MS ^{[1
]}

机构：

[1] UNIV QUEENSLAND, PRINCESS ALEXANDRA HOSP, DEPT MED, BRISBANE, QLD 4102, AUSTRALIA

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 1996年 / 677卷 / 01期

关键词：

phenol; 4-nitrophenol; beta-naphthol;

D O I：

10.1016/0378-4347(95)00368-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This paper describes a simple and more sensitive reversed-phase HPLC method for the quantification of phenol, 4-nitrophenol and beta-naphthol and some of their glucuronide and sulphate conjugates in aqueous solution and liver perfusate buffer. Methanol-water mobile phases with ion-pairing agents for each phenolic group are detailed. The assay showed good recovery, accuracy and precision and is suitable for the quantification of these phenolic compounds in liver perfusion experiments.

引用

页码：117 / 122

页数：6

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