Protein chip fabrication by capture of nascent polypeptides

被引：75

作者：

Tao, Sheng-Ce

Zhu, Heng ^{[1
]}

机构：

[1] Johns Hopkins Univ, Sch Med, Dept Pharmacol & Mol Sci, Baltimore, MD 21205 USA

[2] Johns Hopkins Univ, Sch Med, HiT Ctr, Baltimore, MD 21205 USA

来源：

NATURE BIOTECHNOLOGY | 2006年 / 24卷 / 10期

关键词：

D O I：

10.1038/nbt1249

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The most challenging step in protein microarray fabrication is high-throughput production of proteins. Here we report two similar strategies to fabricate protein chips through capture onto a solid surface of the nascent polypeptides during translation of synthetic or in vitro-transcribed RNAs. Using these approaches, we efficiently fabricated both peptide and protein microarrays at relatively high density. We further demonstrated that such protein chips can be used to analyze protein activity.

引用

页码：1253 / 1254

页数：2

共 14 条

[1] Antibody arrays for high-throughput screening of antibody-antigen interactions [J].

de Wildt, RMT ;

Mundy, CR ;

Gorick, BD ;

Tomlinson, IM .

NATURE BIOTECHNOLOGY, 2000, 18 (09) :989-994

[2] Very low gene duplication rate in the yeast genome [J].

Gao, LZ ;

Innan, H .

SCIENCE, 2004, 306 (5700) :1367-1370

[3]

Haab BB, 2001, GENOME BIOL, V2

[4] Regulation of gene expression by a metabolic enzyme [J].

Hall, DA ;

Zhu, H ;

Zhu, XW ;

Royce, T ;

Gerstein, M ;

Snyder, M .

SCIENCE, 2004, 306 (5695) :482-484

[5] Transcriptional regulatory code of a eukaryotic genome [J].

Harbison, CT ;

Gordon, DB ;

Lee, TI ;

Rinaldi, NJ ;

Macisaac, KD ;

Danford, TW ;

Hannett, NM ;

Tagne, JB ;

Reynolds, DB ;

Yoo, J ;

Jennings, EG ;

Zeitlinger, J ;

Pokholok, DK ;

Kellis, M ;

Rolfe, PA ;

Takusagawa, KT ;

Lander, ES ;

Gifford, DK ;

Fraenkel, E ;

Young, RA .

NATURE, 2004, 431 (7004) :99-104

[6]

Liu RH, 2000, METHOD ENZYMOL, V318, P268

[7]

MacBeath G, 2000, SCIENCE, V289, P1760

[8] The sequence and analysis of duplication-rich human chromosome 16 [J].

Martin, J ;

Han, C ;

Gordon, LA ;

Terry, A ;

Prabhakar, S ;

She, XW ;

Xie, G ;

Hellsten, U ;

Chan, YM ;

Altherr, M ;

Couronne, O ;

Aerts, A ;

Bajorek, E ;

Black, S ;

Blumer, H ;

Branscomb, E ;

Brown, NC ;

Bruno, WJ ;

Buckingham, JM ;

Callen, DF ;

Campbell, CS ;

Campbell, ML ;

Campbell, EW ;

Caoile, C ;

Challacombe, JF ;

Chasteen, LA ;

Chertkov, O ;

Chi, HC ;

Christensen, M ;

Clark, LM ;

Cohn, JD ;

Denys, M ;

Detter, JC ;

Dickson, M ;

Dimitrijevic-Bussod, M ;

Escobar, J ;

Fawcett, JJ ;

Flowers, D ;

Fotopulos, D ;

Glavina, T ;

Gomez, M ;

Gonzales, E ;

Goodstein, D ;

Goodwin, LA ;

Grady, DL ;

Grigoriev, I ;

Groza, M ;

Hammon, N ;

Hawkins, T ;

Haydu, L .

NATURE, 2004, 432 (7020) :988-994

[9] Global analysis of protein phosphorylation in yeast [J].

Ptacek, J ;

Devgan, G ;

Michaud, G ;

Zhu, H ;

Zhu, XW ;

Fasolo, J ;

Guo, H ;

Jona, G ;

Breitkreutz, A ;

Sopko, R ;

McCartney, RR ;

Schmidt, MC ;

Rachidi, N ;

Lee, SJ ;

Mah, AS ;

Meng, L ;

Stark, MJR ;

Stern, DF ;

De Virgilio, C ;

Tyers, M ;

Andrews, B ;

Gerstein, M ;

Schweitzer, B ;

Predki, PF ;

Snyder, M .

NATURE, 2005, 438 (7068) :679-684

[10] Self-assembling protein microarrays [J].

Ramachandran, N ;

Hainsworth, E ;

Bhullar, B ;

Eisenstein, S ;

Rosen, B ;

Lau, AY ;

Walter, JC ;

LaBaer, J .

SCIENCE, 2004, 305 (5680) :86-90

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