HPLC-API/MS/MS: a powerful tool for integrating drug metabolism into the drug discovery process

被引：91

作者：

Korfmacher, WA ^{[1
]}

Cox, KA ^{[1
]}

Bryant, MS ^{[1
]}

Veals, J ^{[1
]}

Ng, K ^{[1
]}

Watkins, R ^{[1
]}

Lin, CC ^{[1
]}

机构：

[1] SCHERING PLOUGH CORP,RES INST,DEPT PHARMACOL,KENILWORTH,NJ 07033

来源：

DRUG DISCOVERY TODAY | 1997年 / 2卷 / 12期

关键词：

D O I：

10.1016/S1359-6446(97)01121-5

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

HPLC combined with atmospheric pressure ionization (API) mass spectrometry (MS) has become a very useful tool in the pharmaceutical industry. The technique of HPLC-API/MS/MS is becoming very important for both drug discovery and drug development programs. In the drug discovery area, it has three major uses: (1) rapid, quantitative method development, (2) metabolite identification, and (3) multi-drug analysis. The sensitivity of the API source and the selectivity provided by tandem mass spectrometry (MS/MS) enable rapid, quantitative method development for drugs in plasma. Early information on the metabolism of candidate drugs can guide structural modifications, thereby improving the activity and/or bioavailability.

引用

页码：532 / 537

页数：10

共 18 条

[1] Allen GD, 1996, LC GC-MAG SEP SCI, V14, P510
[2] ADVANCES IN THE APPLICATION OF MASS-SPECTROMETRY TO STUDIES OF DRUG-METABOLISM, PHARMACOKINETICS AND TOXICOLOGY
BAILLIE, TA
[J]. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY AND ION PROCESSES, 1992, 118 : 289 - 314
[3] BERMAN J, 1997, J MED CHEM, V40, P829
[4] Pharmacokinetic screening for the selection of new drug discovery candidates is greatly enhanced through the use of liquid chromatography atmospheric pressure ionization tandem mass spectrometry
Bryant, MS
Korfmacher, WA
Wang, SY
Nardo, C
Nomeir, AA
Lin, CC
[J]. JOURNAL OF CHROMATOGRAPHY A, 1997, 777 (01) : 61 - 66
[5] CHILTON A, 1997, IN PRESS P 45 ASMS C
[6] DEVELOPMENT AND COMPARISON OF HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHODS WITH TANDEM MASS-SPECTROMETRIC AND ULTRAVIOLET ABSORBENCY DETECTION FOR THE DETERMINATION OF CYCLOBENZAPRINE IN HUMAN PLASMA AND URINE
CONSTANZER, M
CHAVEZ, C
MATUSZEWSKI, B
[J]. JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS, 1995, 666 (01): : 117 - 126
[7] HIGH-SPEED LIQUID-CHROMATOGRAPHY TANDEM MASS-SPECTROMETRY FOR THE DETERMINATION OF DRUGS IN BIOLOGICAL SAMPLES
COVEY, TR
LEE, ED
HENION, JD
[J]. ANALYTICAL CHEMISTRY, 1986, 58 (12) : 2453 - 2460
[8] deHoffmann E, 1996, J MASS SPECTROM, V31, P129
[9] DULIK DM, 1996, MASS SPECTROMETRY BI, P425
[10] BIOMEDICAL AND BIOCHEMICAL APPLICATIONS OF LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY
GELPI, E
[J]. JOURNAL OF CHROMATOGRAPHY A, 1995, 703 (1-2) : 59 - 80

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