Single-cell genome sequencing: current state of the science

被引：1073

作者：

Gawad, Charles ^{[1
,2
]}

Koh, Winston ^{[3
,4
,5
]}

Quake, Stephen R. ^{[3
,4
,5
]}

机构：

[1] St Jude Childrens Res Hosp, Dept Oncol, Memphis, TN 38105 USA

[2] St Jude Childrens Res Hosp, Dept Computat Biol, Memphis, TN 38105 USA

[3] Stanford Univ, Dept Bioengn, Stanford, CA 94304 USA

[4] Stanford Univ, Dept Appl Phys, Stanford, CA 94304 USA

[5] Stanford Univ, Howard Hughes Med Inst, Palo Alto, CA 94304 USA

来源：

NATURE REVIEWS GENETICS | 2016年 / 17卷 / 03期

关键词：

COPY-NUMBER VARIATION; COMPLEX MICROBIAL COMMUNITIES; CIRCULATING TUMOR-CELLS; ACTIVATING MUTATIONS; CLONAL EVOLUTION; AMPLIFICATION; DNA; CANCER; POLYMERASE; MOSAICISM;

D O I：

10.1038/nrg.2015.16

中图分类号：

Q3 [遗传学];

学科分类号：

071007 [遗传学];

摘要：

The field of single-cell genomics is advancing rapidly and is generating many new insights into complex biological systems, ranging from the diversity of microbial ecosystems to the genomics of human cancer. In this Review, we provide an overview of the current state of the field of single-cell genome sequencing. First, we focus on the technical challenges of making measurements that start from a single molecule of DNA, and then explore how some of these recent methodological advancements have enabled the discovery of unexpected new biology. Areas highlighted include the application of single-cell genomics to interrogate microbial dark matter and to evaluate the pathogenic roles of genetic mosaicism in multicellular organisms, with a focus on cancer. We then attempt to predict advances we expect to see in the next few years.

引用

页码：175 / 188

页数：14

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