Methotrexate conjugates: A molecular in vivo protein tag

被引：82

作者：

Miller, LW ^{[1
]}

Sable, J

Goelet, P

Sheetz, MR

Cornish, VW

机构：

[1] Columbia Univ, Dept Chem, New York, NY 10027 USA

[2] Columbia Univ, Dept Biol, New York, NY 10027 USA

[3] Acidophil LLC, La Jolla, CA 92037 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2004年 / 43卷 / 13期

关键词：

D O I：

10.1002/anie.200352852

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The labeling of proteins in living cells with fluorescent substituents is critical to the study of cell biology. A ligand-receptor approach based on the interaction between dihydrofolate reductase (DHFR) and methotrexate (Mtx) serves as a general method for labeling proteins with fluorescent or otherwise functional small molecules in vivo (see figure).

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页码：1672 / 1675

页数：4

相关论文

共 30 条

[1] New biarsenical Ligands and tetracysteine motifs for protein labeling in vitro and in vivo: Synthesis and biological applications [J].

Adams, SR ;

Campbell, RE ;

Gross, LA ;

Martin, BR ;

Walkup, GK ;

Yao, Y ;

Llopis, J ;

Tsien, RY .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (21) :6063-6076

[2]

APPLEMAN JR, 1988, J BIOL CHEM, V263, P9187

[3] INSIGHTS INTO ENZYME FUNCTION FROM STUDIES ON MUTANTS OF DIHYDROFOLATE-REDUCTASE [J].

BENKOVIC, SJ ;

FIERKE, CA ;

NAYLOR, AM .

SCIENCE, 1988, 239 (4844) :1105-1110

[4] Rapidly maturing variants of the Discosoma red fluorescent protein (DsRed) [J].

Bevis, BJ ;

Glick, BS .

NATURE BIOTECHNOLOGY, 2002, 20 (01) :83-87

[5]

BOLIN JT, 1982, J BIOL CHEM, V257, P13650

[6] A monomeric red fluorescent protein [J].

Campbell, RE ;

Tour, O ;

Palmer, AE ;

Steinbach, PA ;

Baird, GS ;

Zacharias, DA ;

Tsien, RY .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (12) :7877-7882

[7] GREEN FLUORESCENT PROTEIN AS A MARKER FOR GENE-EXPRESSION [J].

CHALFIE, M ;

TU, Y ;

EUSKIRCHEN, G ;

WARD, WW ;

PRASHER, DC .

SCIENCE, 1994, 263 (5148) :802-805

[8] Redesigning an FKBP-ligand interface to generate chemical dimerizers with novel specificity [J].

Clackson, T ;

Yang, W ;

Rozamus, LW ;

Hatada, M ;

Amara, JF ;

Rollins, CT ;

Stevenson, LF ;

Magari, SR ;

Wood, SA ;

Courage, NL ;

Lu, XD ;

Cerasoli, F ;

Gilman, M ;

Holt, DA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (18) :10437-10442

[9] Analysis of gene function in somatic mammalian cells using small interfering RNAs [J].

Elbashir, SM ;

Harborth, J ;

Weber, K ;

Tuschl, T .

METHODS, 2002, 26 (02) :199-213

[10] Receptor-mediated targeting of fluorescent probes in living cells [J].

Farinas, J ;

Verkman, AS .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (12) :7603-7606