Expression of CP4 EPSPS in microspores and tapetum cells of cotton (Gossypium hirsutum) is critical for male reproductive development in response to late-stage glyphosate applications

被引:24
作者
Chen, Yun-Chia Sophia [1 ]
Hubmeier, Christopher [1 ]
Tran, Minhtien [1 ]
Martens, Amy [1 ]
Cerny, R. Eric [1 ]
Sammons, R. Doug [1 ]
CaJacob, Claire [1 ]
机构
[1] Monsanto Co, St Louis, MO 63017 USA
关键词
Agrobacterium sp strain CP4 5-enolpyruvylshikimate-3-phosphate synthase (CP4 EPSPS); figwort mosaic virus (FMV) 35S promoter; first position boll (FPB); microspore; pollen mother cell; tapetum;
D O I
10.1111/j.1467-7652.2006.00203.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Plants expressing Agrobacterium sp. strain CP4 5-enolpyruvylshikimate-3-phosphate synthase (CP4 EPSPS) are known to be resistant to glyphosate, a potent herbicide that inhibits the activity of the endogenous plant EPSPS. The RR1445 transgenic cotton line (current commercial line for Roundup Ready((R)) Cotton) was generated using the figwort mosaic virus (FMV) 35S promoter to drive the expression of the CP4 EPSPS gene, and has excellent vegetative tolerance to glyphosate. However, with high glyphosate application rates at developmental stages later than the four-leaf stage (late-stage applications: applications that are inconsistent with the Roundup((R)) labels), RR1445 shows male sterility. Another transgenic cotton line, RR60, was generated using the FMV 35S promoter and the Arabidopsis elongation factor-1 alpha promoter (AtEF1 alpha) for the expression of CP4 EPSPS. RR60 has excellent vegetative and reproductive tolerance to applications of glyphosate at all developmental stages. Histochemical analyses were conducted to examine the male reproductive development at the cellular level of these cotton lines in response to glyphosate applications, and to investigate the correlation between glyphosate injury and the expression of CP4 EPSPS in male reproductive tissues. The expression of CP4 EPSPS in RR60 was found to be strong in all male reproductive cell types. Conversely, CP4 EPSPS expression in RR1445 was low in pollen mother cells, male gametophytes and tapetum, three crucial male reproductive cell types. Our results indicate that the FMV 35S promoter, although expressing strongly in most vegetative tissues in plants, has extremely low activity in these cell types.
引用
收藏
页码:477 / 487
页数:11
相关论文
共 33 条
[1]   DIFFERENTIAL STAINING OF ABORTED AND NONABORTED POLLEN [J].
ALEXANDER, MP .
STAIN TECHNOLOGY, 1969, 44 (03) :117-+
[2]  
Barry G., 1992, CURRENT TOPICS PLANT, P139
[3]  
CaJacob CA, 2004, HDB PLANT BIOTECHNOL, P353
[4]  
Chen YCS, 1996, DEVELOPMENT, V122, P3243
[5]   MODULAR ORGANIZATION AND DEVELOPMENTAL ACTIVITY OF AN ARABIDOPSIS-THALIANA EF-1-ALPHA GENE PROMOTER [J].
CURIE, C ;
AXELOS, M ;
BARDET, C ;
ATANASSOVA, R ;
CHAUBET, N ;
LESCURE, B .
MOLECULAR & GENERAL GENETICS, 1993, 238 (03) :428-436
[6]   CIS AND TRANS-ACTING ELEMENTS INVOLVED IN THE ACTIVATION OF ARABIDOPSIS-THALIANA A1 GENE ENCODING THE TRANSLATION ELONGATION-FACTOR EF-1-ALPHA [J].
CURIE, C ;
LIBOZ, T ;
BARDET, C ;
GANDER, E ;
MEDALE, C ;
AXELOS, M ;
LESCURE, B .
NUCLEIC ACIDS RESEARCH, 1991, 19 (06) :1305-1310
[7]  
DUPUIS I, 1993, PLANT CELL REP, V12, P607, DOI 10.1007/BF00232808
[8]   Glyphosate efficacy is contributed by its tissue concentration and sensitivity in velvetleaf (Abutilon theophrasti) [J].
Feng, PCC ;
Chiu, T ;
Sammons, RD .
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, 2003, 77 (03) :83-91
[9]  
FENNELL A, 1992, PLANT CELL REP, V11, P567, DOI 10.1007/BF00233094
[10]  
FERREIRA KL, 1998, P BELTW COTT C, P848