Prooxidant activity of flavonoids: Copper-dependent strand breaks and the formation of 8-hydroxy-2'-deoxyguanosine in DNA

被引：83

作者：

Yoshino, M

Haneda, M

Naruse, M ^{[1
]}

Murakami, K

机构：

[1] Aichi Med Univ, Dept Biochem, Nagakute, Aichi 4801195, Japan

[2] Aichi Med Univ, Cent Res Labs, Nagakute, Aichi 4801195, Japan

来源：

MOLECULAR GENETICS AND METABOLISM | 1999年 / 68卷 / 04期

关键词：

flavonoid; 8-hydroxy-2'-deoxyguanosine; DNA; copper; hydroxyl radical; DNA strand breaks;

D O I：

10.1006/mgme.1999.2901

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The naturally occurring flavonoids caused strand scission of DNA in the presence of copper ion. Flavonoids such as myricetin, baicalein, and quercetin as well as ascorbic acid cleaved plasmid pBR322 DNA and calf thymus DNA potently. Addition of catalase protected DNA from the strand breaks caused by flavonoids. Treatment of calf thymus DNA with these flavonoids or ascorbate plus copper produced 8-hydroxy-2'-deoxyguanosine. Cuprous ion reduced by flavonoids and ascorbic acid may play a key role in the oxidative cleavage of DNA and the formation of base adduct. Mutagenic and carcinogenic action of flavonoids may be explained by the prooxidant effects of the compounds. (C) 1999 Academic Press.

引用

页码：468 / 472

页数：5

共 23 条

[1] OXIDANTS, ANTIOXIDANTS, AND THE DEGENERATIVE DISEASES OF AGING [J].