Tick-borne encephalitis virus prevalence in Ixodes ricinus ticks collected in high risk habitats of the South-Bohemian region of the Czech Republic

被引:19
作者
Danielová, V [1 ]
Holubová, J [1 ]
Daniel, M [1 ]
机构
[1] Natl Inst Publ Hlth, CZ-10042 Prague 10, Czech Republic
关键词
cell cultures; indirect fluorescent antibody test; infection rate; Ixodes ricinus ticks; tickborne encephalitis virus;
D O I
10.1023/A:1020966605960
中图分类号
Q96 [昆虫学];
学科分类号
摘要
A survey for tick-borne encephalitis virus in Ixodes ricinus ticks was conducted in May 2000 in two districts of the South-Bohemian region of the Czech Republic with a high occurrence of tick-borne encephalitis (TBE) in humans. Homogenized ticks were tested on PS cells, which were examined for any cytopathic effect, plaque assay and indirect fluorescent antibody test (IFT). The IFT has proved to be the most sensitive and most rapid test to demonstrate the virus in ticks. TBE virus was found in 17 of 187 pooled samples, consisting of a total of 2,968 ticks. The mean minimum infection rate was 0.6% for all tick stages combined. Infection rates in nymphs collected in different locations varied between 0.2 and 1.3% and between 5.9 and 11.1% in adult ticks. The observed TBE prevalence in ticks was compared with data obtained elsewhere in the Czech Republic. It is concluded that screening of ticks for TBE virus prevalence using IFT is a valuable indicator for the degree of risk to contract TBE in as particular habitat.
引用
收藏
页码:145 / 151
页数:7
相关论文
共 7 条
[1]   Predictive map of Ixodes vicinus high-incidence habitats and a tick-borne encephalitis risk assessment using satellite data [J].
Daniel, M ;
Kolar, J ;
Zeman, P ;
Pavelka, K ;
Sadlo, J .
EXPERIMENTAL & APPLIED ACAROLOGY, 1998, 22 (07) :417-433
[2]  
DANIEL M, 2000, REMEDIA CLIN MICROBI, V4, P252
[3]  
DANIELOVA V, 1983, FOLIA PARASIT, V30, P153
[4]  
Danielova V., 1980, NEW ASPECTS ECOLOGY, P445
[5]  
JANUSKA J, 2000, IN PRESS RESULTS VIR
[6]  
Süss J, 1999, ZBL BAKT-INT J MED M, V289, P564
[7]  
2001, NOTIFICATION INFECT