Differential expression of ileal Na+/H+ exchanger isoforms after enterectomy

被引:16
作者
Falcone, RA [1 ]
Shin, CE
Stern, LE
Wang, ZH
Erwin, CR
Soleimani, M
Warner, BW
机构
[1] Childrens Hosp, Med Ctr, Dept Surg, Div Pediat Surg, Cincinnati, OH 45229 USA
[2] Univ Cincinnati, Coll Med, Dept Med, Div Nephrol, Cincinnati, OH 45229 USA
关键词
intestinal resection; adaptation; mouse; transport;
D O I
10.1006/jsre.1999.5720
中图分类号
R61 [外科手术学];
学科分类号
摘要
Background, Na+/H+ exchangers (NHE) are transporters involved in the absorption of NaCl along the gastrointestinal tract. The aim of this study was to determine the expression pattern of the intestinal brush border NHE isoforms 2 and 3 following massive small bowel resection (SBR). Additionally, the effect of epidermal growth factor (EGF) and salivarectomy (removal of the primary source of EGF) on the expression pattern was studied. Materials and methods. ICR mice underwent a proximal SBR or sham surgery and then received either orogastric saline or EGF (50 mu g/kg/day). In separate experiments mice underwent salivarectomy followed by SBR or sham. Postoperatively the remaining ileum was isolated and levels of NHE-2 and NHE-3 mRNA and protein were resolved. Results. Following SBR, the expression of both mRNA and protein for NHE-3 increased by similar to 2.5-fold. Treatment with EGF enhanced NHE-3 mRNA in sham animals with further elevation following SBR. The expression of NHE-2 mRNA demonstrated minimal change while protein. marginally increased (40%) following SER, EGF did not affect the expression of NHE-2 mRNA. Salivarectomy did not influence NHE-2 protein expression and inhibited the increased NHE-3 protein expression following SBR. Conclusions. Following SBR, the expression pattern for brush border NHE isoforms is distinctive. Increased expression of NHE-3 secondary to SBR and/or EGF treatment with loss of this increase following salivarectomy implies a common mechanism to-enhance enterocyte proliferation and luminal absorption of NaCl and water. These results suggest that NHE-3 is an important ileal exchanger following SBR. (C) 1999 Academic Press.
引用
收藏
页码:192 / 197
页数:6
相关论文
共 29 条
[1]  
ALLEMAIN G, 1984, J BIOL CHEM, V259, P4313
[2]  
AMLAL H, 1997, J AM SOC NEPHROL, V8, pA2
[3]   EPIDERMAL GROWTH-FACTOR ENHANCES INTESTINAL ADAPTATION AFTER MASSIVE SMALL-BOWEL RESECTION [J].
CHAET, MS ;
ARYA, G ;
ZIEGLER, MM ;
WARNER, BW .
JOURNAL OF PEDIATRIC SURGERY, 1994, 29 (08) :1035-1039
[4]   Sodium/hydrogen exchanger gene defect in slow-wave epilepsy mutant mice [J].
Cox, GA ;
Lutz, CM ;
Yang, CL ;
Biemesderfer, D ;
Bronson, RT ;
Fu, A ;
Aronson, PS ;
Noebels, JL ;
Frankel, WN .
CELL, 1997, 91 (01) :139-148
[5]  
GHISHAN FK, 1992, P SOC EXP BIOL MED, V201, P289
[6]   NA+/H+ EXCHANGE AND GROWTH FACTOR-INDUCED CYTOSOLIC PH CHANGES - ROLE IN CELLULAR PROLIFERATION [J].
GRINSTEIN, S ;
ROTIN, D ;
MASON, MJ .
BIOCHIMICA ET BIOPHYSICA ACTA, 1989, 988 (01) :73-97
[7]   Adaptation after small bowel resection is attenuated by sialoadenectomy: The role for endogenous epidermal growth factor [J].
Helmrath, MA ;
Shin, CE ;
Fox, JW ;
Erwin, CR ;
Warner, BW .
SURGERY, 1998, 124 (05) :848-854
[8]   Intestinal adaptation is enhanced by epidermal growth factor independent of increased ileal epidermal growth factor receptor expression [J].
Helmrath, MA ;
Shin, CE ;
Erwin, CR ;
Warner, BW .
JOURNAL OF PEDIATRIC SURGERY, 1998, 33 (07) :980-984
[9]   A defective EGF-receptor in waved-2 mice attenuates intestinal adaptation [J].
Helmrath, MA ;
Erwin, CR ;
Warner, BW .
JOURNAL OF SURGICAL RESEARCH, 1997, 69 (01) :76-80
[10]   Epidermal growth factor upregulates the expression of its own intestinal receptor after small bowel resection [J].
Helmrath, MA ;
Shin, CE ;
Erwin, CR ;
Warner, BW .
JOURNAL OF PEDIATRIC SURGERY, 1998, 33 (02) :229-233