Toxicity and bioavailability to bacteria of particle-associated arsenite and mercury

被引:30
作者
Petänen, T
Romantschuk, M
机构
[1] Univ Helsinki, Div Gen Microbiol, Dept Biosci, FIN-00014 Helsinki, Finland
[2] Univ Helsinki, Dept Ecol & Environm Sci, FIN-15140 Lahti, Finland
关键词
biosensor; biomobilisation; heavy metals; soil; sediment; Pseudomonas fluorescens;
D O I
10.1016/S0045-6535(02)00505-2
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The overall toxicity of soil, and the bioavailability and arsenite from soil were measured with the constructed constitutively luminescent strain Pseudomonas fluorescens OS8 (pNEP01) and with earlier published biosensor strains P. fluorescens OS8 (pTPT11) for mercury and P. fluorescens OS8 (pTPT31) for arsenite, respectively. Both spiked and authentic samples were studied: By combining bacterial assays enabled partial analysis of reasons for toxicity of environmental samples, some of which were highly toxic despite containing little or no heavy metals. The spiked soils were not toxic overall but the method of measuring concentration from water-extractable fraction or from soil-water slurry affected the results significantly. Mercury that was bound to clay even after water extraction was nevertheless found to be bioavailable to a high degree to the biosensor bacteria. Since induction of the luminescence genes takes place intracellularly the bacteria may able to apparently release mercury when in direct contact with clay particle. This type of biornobilisation was not observed with arsenite spiked soils. The same phenomenon was detected in one of the environmental samples. (C) 2002 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:409 / 413
页数:5
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