Contribution of mast cells to the T helper 2 response induced by simultaneous subcutaneous and oral immunization

被引:10
作者
Aoki, I
Itoh, S
Yokota, S
Tanaka, SI
Ishii, N
Okuda, K
Minami, M
Klinman, DM
机构
[1] Yokohama City Univ, Sch Med, Dept Pathol, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan
[2] Yokohama City Univ, Sch Med, Dept Pediat, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan
[3] Yokohama City Univ, Sch Med, Dept Internal Med, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan
[4] Yokohama City Univ, Sch Med, Dept Dermatol, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan
[5] Yokohama City Univ, Sch Med, Dept Bacteriol, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan
[6] Yokohama City Univ, Sch Med, Dept Parasitol, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan
[7] US FDA, Sect Retroviral Immunol, Div Viral Prod, Ctr Biol Evaluat & Res, Bethesda, MD 20014 USA
关键词
D O I
10.1046/j.1365-2567.1999.00878.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
This work examines the contribution of mast cells to the synergistic enhancement of the T helper 2 (Th2) immune response elicited following simultaneous oral and subcutaneous (s.c.) immunization. The s.c. route induced a Th1-biased immune response, characterized by increased interferon-gamma (IFN-gamma) and immunoglobulin G2a (IgG2a) antibody production. In contrast, oral immunization stimulated a primarily Th2-type response in which interleukin-4 (IL-4) and IgG1 antibody production were dominant. Simultaneous immunization also triggered a Th2-biased response, the magnitude of which exceeded the additive effects of s.c. and oral immunization alone by greater than threefold. To analyse whether mast cells in gut-associated lymphoid tissue contributed to this synergistic response, mast cell-deficient mice WBB6F(1)-w/w(v) were studied. Whereas the primary response following simultaneously antigen administration was reduced only twofold in these animals compared with wild type controls WBB6F(1)-+/+ (suggesting that mast cells were not needed to initiate Th2 immunity), reconstitution with bone-marrow-derived mast cells from WBB6F1-+/+ mice resulted in a superoptimal response (suggesting that mast cells contribute to the magnitude and perpetuation of these Th2-biased responses).
引用
收藏
页码:519 / 524
页数:6
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