FXYD7 is a brain-specific regulator of Na,K-ATPase α1-β isozymes

被引:106
作者
Béguin, P
Crambert, G
Monnet-Tschudi, F
Uldry, M
Horisberger, JD
Garty, H
Geering, K
机构
[1] Univ Lausanne, Inst Pharmacol & Toxicol, CH-1005 Lausanne, Switzerland
[2] Univ Lausanne, Inst Physiol, CH-1005 Lausanne, Switzerland
[3] Weizmann Inst Sci, Dept Biol Chem, IL-76100 Rehovot, Israel
关键词
brain Na; K-ATPase modulator; FXYD7; FXYD proteins; Xenopus oocytes;
D O I
10.1093/emboj/cdf330
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Recently, corticosteroid hormone-induced factor (CHIF) and the gamma-subunit, two members of the FXYD family of small proteins, have been identified as regulators of renal Na,K-ATPase. In this study, we have investigated the tissue distribution and the structural and functional properties of FXYD7, another family member which has not yet been characterized. Expressed exclusively in the brain, FXYD7 is a type I membrane protein bearing N-terminal, post-translationally added modifications on threonine residues, most probably O-glycosylations that are important for protein stabilization. Expressed in Xenopus oocytes, FXYD7 can interact with Na,K-ATPase alpha1-beta1, alpha2-beta1 and alpha3-beta1 but not with alpha-beta2 isozymes, whereas, in brain, it is only associated with alpha1-beta isozymes. FXYD7 decreases the apparent K+ affinity of alpha1-beta1 and alpha2-beta1, but not of alpha3-beta1 isozymes. These data suggest that FXYD7 is a novel, tissue- and isoform-specific Na,K-ATPase regulator which could play an important role in neuronal excitability.
引用
收藏
页码:3264 / 3273
页数:10
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