Expression of Kaposi's sarcoma-associated herpesvirus-encoded K10/10.1 protein in tissues and its interaction with poly(A)-binding protein

被引：31

作者：

Kanno, Takayuki ^{[1
]}

Sato, Yuko ^{[1
]}

Sata, Tetsutaro ^{[1
]}

Katano, Harutaka ^{[1
]}

机构：

[1] Natl Inst Infect Dis, Dept Pathol, Shinjuku Ku, Tokyo 1628640, Japan

来源：

VIROLOGY | 2006年 / 352卷 / 01期

关键词：

Kaposi's sarcoma-associated herpesvirus (KSHV/HHV-8); K10/10; 1; Kaposi's sarcoma (KS); Multicentric Castleman's disease (MCD); poly(A)-binding protein (PABP); primary effusion lymphoma (PEL);

D O I：

10.1016/j.virol.2006.04.009

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The K 10/10.1 protein is encoded by a cluster of interferon regulatory factor (IRF) homologues in the Kaposi's sarcoma-associated herpesvirus (KSHV, human herpesvirus 8, HHV-8) genome. In the present study, we showed that an anti-KIO antibody reacted with a 110-kDa protein encoded by the K10/10.1 gene of KSHV in KSHV-infected primary effusion lymphoma (PEL) cell lines. Expression of K10/10.1 protein was induced by phorbol ester in KSHV-infected cells. A reporter gene assay demonstrated that K10/10.1 protein did not influence promoter activity of human interferon genes, regardless of its homology to human IRFs. Poly(A)-binding protein (PABP) was identified as a partner of K10/10.1 protein. Immunoprecipitation revealed that K10/10.1 protein interacted with PABP specifically in PEL cell lines. IFA revealed co-localization of K10/10.1 protein and PABP in the nucleus of KSHV-infected cells. These data suggest that K10/10.1 protein may affect the translational status or stability of mRNA in host cells. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：100 / 109

页数：10

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