Clonal propagation of primate offspring by embryo splitting

被引：84

作者：

Chan, AWS

Dominko, T

Luetjens, CM

Neuber, E

Martinovich, C

Hewitson, L

Simerly, CR

Schatten, GP ^{[1
]}

机构：

[1] Oregon Reg Primate Res Ctr, Beaverton, OR 97006 USA

[2] Oregon Hlth & Sci Univ, Dept Obstet & Gynecol, Portland, OR 97201 USA

[3] Oregon Hlth & Sci Univ, Dept Cell & Dev Biol, Portland, OR 97201 USA

来源：

SCIENCE | 2000年 / 287卷 / 5451期

关键词：

D O I：

10.1126/science.287.5451.317

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Primates that are identical in both nuclear and cytoplasmic components have not been produced by current cloning strategies, yet such identicals represent the ideal model for investigations of human diseases. Here, genetically identical nonhuman embryos were produced as twin and larger sets by separation and reaggregation of blastomeres of cleavage-stage embryos. A total of 368 multiples were created by the splitting of 107 rhesus embryos with four pregnancies established after 13 embryo transfers (31% versus 53% in vitro fertilization controls). The birth of Tetra, a healthy female cloned from a quarter of an embryo, proves that this approach can result in live offspring.

引用

收藏

页码：317 / 319

页数：3

相关论文

共 28 条

[1] Production of goats by somatic cell nuclear transfer [J].

Baguisi, A ;

Behboodi, E ;

Melican, DT ;

Pollock, JS ;

Destrempes, MM ;

Cammuso, C ;

Williams, JL ;

Nims, SD ;

Porter, CA ;

Midura, P ;

Palacios, MJ ;

Ayres, SL ;

Denniston, RS ;

Hayes, ML ;

Ziomek, CA ;

Meade, HM ;

Godke, RA ;

Gavin, WG ;

Overström, EW ;

Echelard, Y .

NATURE BIOTECHNOLOGY, 1999, 17 (05) :456-461

[2] Cloned transgenic calves produced from nonquiescent fetal fibroblasts [J].

Cibelli, JB ;

Stice, SL ;

Golueke, PJ ;

Kane, JJ ;

Jerry, J ;

Blackwell, C ;

de Leon, FAP ;

Robl, JM .

SCIENCE, 1998, 280 (5367) :1256-1258

[3] IMPLANTATION ENHANCEMENT BY SELECTIVE ASSISTED HATCHING USING ZONA DRILLING OF HUMAN EMBRYOS WITH POOR PROGNOSIS [J].

COHEN, J ;

ALIKANI, M ;

TROWBRIDGE, J ;

ROSENWAKS, Z .

HUMAN REPRODUCTION, 1992, 7 (05) :685-691

[4] Mitochondrial DNA genotypes in nuclear transfer-derived cloned sheep [J].

Evans, MJ ;

Gurer, C ;

Loike, JD ;

Wilmut, I ;

Schnieke, AE ;

Schon, EA .

NATURE GENETICS, 1999, 23 (01) :90-93

[5]

Gardner RL, 1997, DEVELOPMENT, V124, P289

[6] Maternal thyroid deficiency during pregnancy and subsequent neuropsychological development of the child [J].

Haddow, JE ;

Palomaki, GE ;

Allan, WC ;

Williams, JR ;

Knight, GJ ;

Gagnon, J ;

O'Heir, CE ;

Mitchell, ML ;

Hermos, RJ ;

Waisbren, SE ;

Faix, JD ;

Klein, RZ .

NEW ENGLAND JOURNAL OF MEDICINE, 1999, 341 (08) :549-555

[7] Fertilization and embryo development to blastocysts after intracytoplasmic sperm injection in the rhesus monkey [J].

Hewitson, L ;

Takahashi, D ;

Dominko, T ;

Simerly, C ;

Schatten, G .

HUMAN REPRODUCTION, 1998, 13 (12) :3449-3455

[8] Unique checkpoints during the first cell cycle of fertilization after intracytoplasmic sperm injection in rhesus monkeys [J].

Hewitson, L ;

Dominko, T ;

Takahashi, D ;

Martinovich, C ;

Ramalho-Santos, J ;

Sutovsky, P ;

Fanton, J ;

Jacob, D ;

Monteith, D ;

Neuringer, M ;

Battaglia, D ;

Simerly, C ;

Schatten, G .

NATURE MEDICINE, 1999, 5 (04) :431-433

[9] Cloning in cattle:: from embryo splitting to somatic nuclear transfer [J].

Heyman, Y ;

Vignon, X ;

Chesné, P ;

Le Bourhis, D ;

Marchal, J ;

Renard, JP .

REPRODUCTION NUTRITION DEVELOPMENT, 1998, 38 (06) :595-603

[10] Clinical and pathologic features of cloned transgenic calves and fetuses (13 case studies) [J].

Hill, JR ;

Roussel, AJ ;

Cibelli, JB ;

Edwards, JF ;

Hooper, NL ;

Miller, MW ;

Thompson, JA ;

Looney, CR ;

Westhusin, ME ;

Robl, JM ;

Stice, SL .

THERIOGENOLOGY, 1999, 51 (08) :1451-1465