transgenic mice;
saturated phosphatidylcholine;
surfactant protein A;
surfactant protein D;
alveolar proteinosis;
D O I:
10.1152/ajplung.2000.278.1.L75
中图分类号:
Q4 [生理学];
学科分类号:
071003 ;
摘要:
IL-4 increases surfactant and regulates metabolism in vivo. Am. J. Physiol. Lung Cell. Mel. Physiol. 278: L75-L80, 2000.-Mice that express interleukin (IL)-4 in Clara cells (CCSP-IL-4) develop chronic airway inflammation and an alveolar proteinosis-like syndrome. To identify the role of IL-4 in surfactant homeostasis, we measured lipid and protein metabolism in the lungs of CCSP-IL-4 mice in vivo. Alveolar saturated phosphatidylcholine (Sat PC) pools were increased 6.5-fold and lung tissue Sat PC pools were increased 4.8-fold in the IL-4 transgenic mice. Whereas surfactant protein (SP) A was increased proportionately to Sat PC, SP-D was increased approximately 90-fold in the IL-4 mice compared with wild-type mice and was associated with 2.8-fold increase in SP-D mRNA. The incorporation of palmitate and choline into Sat PC was increased about twofold in CCSP-IL-4 mice. Although trace doses of radiolabeled Sat PC were cleared from the air spaces and lungs of CCSP-IL-4 mice more slowly than in wild-type mice, net clearance of Sat PC from the lungs of CCSP-IL-4 mice was sixfold higher in the IL-4 mice than in wild-type mice because of the larger Sat PC pool sizes. Expression of IL-4 in Clara cells increased surfactant lipid synthesis and clearance, establishing a new equilibrium with increased surfactant pools and an alveolar proteinosis associated with a selective increase in SP-D protein, demonstrating a previously unexpected effect of IL-4 in pulmonary surfactant homeostasis.
机构:
Washington Univ, Barnes Jewish Hosp, Med Ctr, Dept Pathol, St Louis, MO 63110 USAWashington Univ, Barnes Jewish Hosp, Med Ctr, Dept Pathol, St Louis, MO 63110 USA
机构:
Catholic Univ Louvain, Fac Med, Unit Ind Toxicol & Occupat Med, B-1200 Brussels, BelgiumCatholic Univ Louvain, Fac Med, Unit Ind Toxicol & Occupat Med, B-1200 Brussels, Belgium
Hermans, C
;
Bernard, A
论文数: 0引用数: 0
h-index: 0
机构:
Catholic Univ Louvain, Fac Med, Unit Ind Toxicol & Occupat Med, B-1200 Brussels, BelgiumCatholic Univ Louvain, Fac Med, Unit Ind Toxicol & Occupat Med, B-1200 Brussels, Belgium
机构:
Washington Univ, Barnes Jewish Hosp, Med Ctr, Dept Pathol, St Louis, MO 63110 USAWashington Univ, Barnes Jewish Hosp, Med Ctr, Dept Pathol, St Louis, MO 63110 USA
机构:
Catholic Univ Louvain, Fac Med, Unit Ind Toxicol & Occupat Med, B-1200 Brussels, BelgiumCatholic Univ Louvain, Fac Med, Unit Ind Toxicol & Occupat Med, B-1200 Brussels, Belgium
Hermans, C
;
Bernard, A
论文数: 0引用数: 0
h-index: 0
机构:
Catholic Univ Louvain, Fac Med, Unit Ind Toxicol & Occupat Med, B-1200 Brussels, BelgiumCatholic Univ Louvain, Fac Med, Unit Ind Toxicol & Occupat Med, B-1200 Brussels, Belgium