Cloning of genes associated with the colonization of tomato roots by the arbuscular mycorrhizal fungus Glomus mosseae

Complimentary DNA (cDNA) libraries were made from RNA isolated from uninoculated (control) tomato roots and tomato roots colonized with arbuscular mycorrhizal fungus Glomus mosseae. Four clones of genes upregulated, and one clone of a gene downregulated by G mosseae colonization of roots were isolated by differential screening. Partial sequencing of the cDNA clones revealed that the upregulated clones showed significant similarities to the following genes: a phosphoenolpyruvate carboxylase gene from nodules of alfalfa, a human cullin gene, a nematode-induced gene in giant cells of tomato roots and a mannitol dehydrogenase gene from celery and parsley. The clone of the repressed gene did not show any significant similarity to known sequences in the databases.