Recent advances on coherent anti-stokes Raman scattering (CARS) microscopy

被引：1

作者：

Cheng, JX ^{[1
]}

Potma, EO ^{[1
]}

Xie, SNX ^{[1
]}

机构：

[1] Harvard Univ, Dept Chem & Biol Chem, Cambridge, MA 02138 USA

来源：

MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES II | 2002年 / 4620卷

关键词：

CARS; vibrational imaging; NIH3T3; cells; mitosis; apoptosis;

D O I：

10.1117/12.470697

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Recent advances on CARS microscopy provided exciting possibilities for imaging living cells with high sensitivity, high 3D spatial resolution and chemical selectivity based on vibrational spectroscopy. Epi-detection avoids the forward-going water signal and allows imaging of small features inside cells. Polarization-sensitive detection improves the vibrational contrast in CARS microscopy via suppression of the non-resonant background. High-speed CARS imaging of living cells is realized by raster scanning two collinearly overlapped near infrared picosecond laser beams. Vibrational mapping of lipids and proteins inside living cells is achieved. CARS imaging of unstained cells during mitosis and apoptosis is carried out. Incorporation of CARS microscopy with the photon correlation technique allows probing diffusion dynamics of 50-nm beads with chemical selectivity.

引用

页码：248 / 258

页数：11

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