Diagnosis of pelvic actinomycosis by 16S ribosomal RNA gene sequencing and its clinical significance

被引:37
作者
Woo, PCY
Fung, AMY
Lau, SKP
Hon, E
Yuen, KY [1 ]
机构
[1] Univ Hong Kong, Queen Mary Hosp, Dept Microbiol, Hong Kong, Hong Kong, Peoples R China
[2] HKU, Pasteur Res Ctr, Hong Kong, Hong Kong, Peoples R China
[3] Univ Hong Kong, Queen Mary Hosp, Dept Obstet & Gynaecol, Hong Kong, Hong Kong, Peoples R China
关键词
D O I
10.1016/S0732-8893(02)00375-9
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Traditional ways of identification of anaerobic Gram-positive non-sporulating bacilli by isolation of the organism and studying it phenotypically by elucidation of its morphologic and biochemical characteristics and metabolic end products are associated with a need for special equipment and expertise, and strains that are "unidentified" because of ambiguous biochemical profiles. In this study, an anaerobic Gram-positive non-sporulating bacterium was isolated from the intrauterine contraceptive device of a 36-year old woman with pyosalpinx. The Vitek system (ANI) showed that it was 99% Propionibacterium graindosum; whereas the API system (20A) showed that it was 78% Actinomyces meyeri/odontolyticus. The 16S ribosomal RNA gene of the strain was amplified and sequenced. There was 0 base difference between the isolate and A. odontolyticus (GenBank Accession no. AJ234047), indicating the isolate most closely resembled a strain of A. odontolyticus. Identification of the organism in this study was important because the duration of antibiotic therapy would be entirely different. In the present case, identification of the bacterium as A. odontolyticus inferred that the patient suffered from an intermediate form of pelvic actinomycosis. A prolonged course of antibiotics would be more desirable, as the relapse rate of actinomycosis after a short course of antibiotics is high. (C) 2002 Elsevier Science Inc. All rights reserved.
引用
收藏
页码:113 / 118
页数:6
相关论文
共 29 条
[1]   SALPINGITIS - ASPECTS OF DIAGNOSIS AND ETIOLOGY - A 4-YEAR STUDY FROM A SWEDISH CAPITAL HOSPITAL [J].
BRIHMER, C ;
KALLINGS, I ;
NORD, CE ;
BRUNDIN, J .
EUROPEAN JOURNAL OF OBSTETRICS GYNECOLOGY AND REPRODUCTIVE BIOLOGY, 1987, 24 (03) :211-220
[2]  
Cheuk W, 2000, J PATHOL, V192, P289, DOI 10.1002/1096-9896(2000)9999:9999&lt
[3]  
::AID-PATH767&gt
[4]  
3.0.CO
[5]  
2-F
[6]   ENTEROBACTER BACTEREMIA - CLINICAL-FEATURES AND EMERGENCE OF ANTIBIOTIC-RESISTANCE DURING THERAPY [J].
CHOW, JW ;
FINE, MJ ;
SHLAES, DM ;
QUINN, JP ;
HOOPER, DC ;
JOHNSON, MP ;
RAMPHAL, R ;
WAGENER, MM ;
MIYASHIRO, DK ;
YU, VL .
ANNALS OF INTERNAL MEDICINE, 1991, 115 (08) :585-590
[7]   SYSTEMIC ACTINOMYCES INFECTION - A POTENTIAL COMPLICATION OF INTRAUTERINE CONTRACEPTIVE DEVICES [J].
DELAMONTE, SM ;
GUPTA, PK ;
WHITE, CL .
JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1982, 248 (15) :1876-1877
[8]   BILATERAL FEMALE PELVIC ACTINOMYCOSIS [J].
HANSEN, LK .
ACTA OBSTETRICIA ET GYNECOLOGICA SCANDINAVICA, 1989, 68 (02) :189-190
[9]  
HILL GB, 1992, OBSTET GYNECOL, V79, P534
[10]   CHARACTERISTICS AND SITES OF INFECTION OF EUBACTERIUM-NODATUM, EUBACTERIUM-TIMIDUM, EUBACTERIUM-BRACHY, AND OTHER ASACCHAROLYTIC EUBACTERIA [J].
HILL, GB ;
AYERS, OM ;
KOHAN, AP .
JOURNAL OF CLINICAL MICROBIOLOGY, 1987, 25 (08) :1540-1545