Urinary excretion of the collagen cross-link pyridinoline increases during liver fibrogenesis

被引:10
作者
Grenard, P [1 ]
Blanquier, B [1 ]
RicardBlum, S [1 ]
机构
[1] INST PASTEUR LYON,CNRS URA 1459,F-69365 LYON 07,FRANCE
关键词
collagen; cross-linking; liver fibrosis; pyridinoline; schistosomiasis;
D O I
10.1016/S0168-8278(97)80472-2
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Background/Aims: Pyridinoline, a specific cross-link of mature collagen, increases in liver during fibrogenesis and its hepatic level is related to the degree of reversibility of the fibrotic process, Since pyridinoline is excreted in urine, we have investigated the relationship between its urinary level and liver fibrogenesis in a model of mild and reversible liver fibrosis, murine schistosomiasis. Methods: Pyridinoline was measured by HPLC in urine and in liver of Schistosoma mansoni-infected mice during the acute and the chronic phases of the infection, Collagen deposition was measured colorimetrically, Both the isolated granulomas and the surrounding liver parenchyma were analyzed. Results: In infected mice, pyridinoline increased mainly in the isolated granulomas, corresponding to the fibrotic lesions, and slightly in the surrounding parenchyma, The urinary excretion of pyridinoline increased during liver fibrogenesis and was correlated to the duration of infection (r=0.81) and to the collagen content of granulomas (r=0.81). The treatment of infected mice by praziquantel, an antiparasitic drug, did not lead to significant changes in liver collagen cross-linking by pyridinoline either in granulomas or in parenchyma, The major effect of the drug was targeted at the collagen content of parenchyma, which decreased by 50%, 18 weeks after treatment, The uninary level of pyridinoline of treated mice nas negatively correlated to the length of the treatment follow-up (r=-0.76). Conclusions: The measurement of the urinary excretion of pyridinoline could be helpful to monitor the remodeling of liver extracellular matrix occurring in fibrogenesis and the effect of chemotherapy.
引用
收藏
页码:1356 / 1362
页数:7
相关论文
共 29 条
[1]  
ANDRADE ZA, 1986, AM J PATHOL, V124, P59
[2]   QUANTITATIVE-ANALYSIS OF THE PYRIDINIUM CROSSLINKS OF COLLAGEN IN URINE USING ION-PAIRED REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].
BLACK, D ;
DUNCAN, A ;
ROBINS, SP .
ANALYTICAL BIOCHEMISTRY, 1988, 169 (01) :197-203
[3]   IMMUNOPATHOLOGY OF SCHISTOSOMA-MANSONI INFECTION [J].
BOROS, DL .
CLINICAL MICROBIOLOGY REVIEWS, 1989, 2 (03) :250-269
[4]  
BRINDLEY PJ, 1987, J IMMUNOL, V139, P215
[5]   RELATIONSHIPS BETWEEN CHEMOTHERAPY AND IMMUNITY IN SCHISTOSOMIASIS [J].
BRINDLEY, PJ .
ADVANCES IN PARASITOLOGY, VOL 34, 1994, 34 :133-161
[6]   PERSISTENCE OF EGGS AND HEPATIC-FIBROSIS AFTER TREATMENT OF SCHISTOSOMA-MANSONI-INFECTED MICE [J].
CHEEVER, AW ;
MACEDONIA, JG ;
ERIK, SD ;
CHEEVER, EA ;
MOSIMANN, JE .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1992, 46 (06) :752-758
[7]   NEW CHALLENGES IN HEPATIC-FIBROSIS [J].
CLEMENT, B ;
LOREAL, O ;
LEVAVASSEUR, F ;
GUILLOUZO, A .
JOURNAL OF HEPATOLOGY, 1993, 18 (01) :1-4
[8]   FACTORS AFFECTING THE ASSAY OF URINARY 3-HYDROXY PYRIDINIUM CROSS-LINKS OF COLLAGEN AS MARKERS OF BONE-RESORPTION [J].
COLWELL, A ;
RUSSELL, RGG ;
EASTELL, R .
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION, 1993, 23 (06) :341-349
[9]  
GRESSNER AM, 1991, EUR J CLIN CHEM CLIN, V29, P293
[10]   COLLAGEN CROSS-LINKING COMPOUNDS IN HUMAN-URINE [J].
GUNJASMITH, Z ;
BOUCEK, RJ .
BIOCHEMICAL JOURNAL, 1981, 197 (03) :759-762