Genome engineering empowers the diatom Phaeodactylum tricornutum for biotechnology

被引：309

作者：

Daboussi, Fayza ^{[1
]}

Leduc, Sophie ^{[1
]}

Marechal, Alan ^{[1
]}

Dubois, Gwendoline ^{[1
]}

Guyot, Valerie ^{[1
]}

Perez-Michaut, Christophe ^{[1
]}

Amato, Alberto ^{[1
]}

Falciatore, Angela ^{[2
,3
]}

Juillerat, Alexandre ^{[1
]}

Beurdeley, Marine ^{[1
]}

Voytas, Daniel F. ^{[4
]}

Cavarec, Laurent ^{[1
]}

Duchateau, Philippe ^{[1
]}

机构：

[1] Cellectis SA, F-75013 Paris, France

[2] Univ Paris 06, CNRS, UMR7238, F-75006 Paris, France

[3] CNRS, UMR7238, Lab Biol Computat & Quantitat, F-75006 Paris, France

[4] Cellectis Plant Sci, New Brighton, MN 55112 USA

来源：

NATURE COMMUNICATIONS | 2014年 / 5卷

关键词：

LIPID-ACCUMULATION; HOMOLOGOUS RECOMBINATION; HOMING ENDONUCLEASE; HIGH-EFFICIENCY; GENE-THERAPY; METABOLISM; MICROALGAE; PERSPECTIVES; PATHWAYS; GROWTH;

D O I：

10.1038/ncomms4831

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Diatoms, a major group of photosynthetic microalgae, have a high biotechnological potential that has not been fully exploited because of the paucity of available genetic tools. Here we demonstrate targeted and stable modifications of the genome of the marine diatom Phaeodactylum tricornutum, using both meganucleases and TALE nucleases. When nuclease-encoding constructs are co-transformed with a selectable marker, high frequencies of genome modifications are readily attained with 56 and 27% of the colonies exhibiting targeted mutagenesis or targeted gene insertion, respectively. The generation of an enhanced lipid-producing strain (45-fold increase in triacylglycerol accumulation) through the disruption of the UDP-glucose pyrophosphorylase gene exemplifies the power of genome engineering to harness diatoms for biofuel production.

引用

页数：7

共 44 条

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