Recovery of a novel Ca-binding peptide from Alaska Pollack (Theragra chalcogramma) backbone by pepsinolytic hydrolysis

被引：113

作者：

Jung, Won-Kyo

Karawita, Rohan

Heo, Soo-Jin

Lee, Bae-Jin

Kim, Se-Kwon

Jeon, You-Jin ^{[1
]}

机构：

[1] Jeju Natl Univ, Fac Appl Marine Sci, Cheju 690756, South Korea

[2] Pukyong Natl Univ, Dept Chem, Pusan 608737, South Korea

[3] Pukyong Natl Univ, Marine Bioproc Co Ltd, Pusan 608737, South Korea

来源：

PROCESS BIOCHEMISTRY | 2006年 / 41卷 / 09期

关键词：

Alaska Pollack (Theragra chalcogramma) backbone; pepsinolytic hydrolysis; hydroxyapaptite; calcium-binding peptide;

D O I：

10.1016/j.procbio.2006.05.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Low molecular weight peptide with a high affinity to calcium was recovered from pepsinolytic hydrolysates of Theragra chalcogramma backbone discarded from fish processing using a hydroxyapatite affinity chromatography. T chalcogramma backbone peptide (TBP), Val-Leu-SerGly-Gly-Thr-Thr-Met-Ala-Met-Tyr-Thr-Leu-VaI (Mw: 1442 Da), exhibited the highest affinity to calcium ion on the surface of hydroxyapaptite (HA) crystals. In vitro calcium-binding assay elucidated that TBP can solublize a similar content of calcium with casein phosphopeptide (CPP). In the present study, it is possible to provide utilization of fish backbones in a novel nutraccutical material with a high solubility for calcium to oriental people with lactose indigestion and intolerance. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：2097 / 2100

页数：4

共 16 条

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