Graphite epoxy composite electrodes modified with bacterial cells

被引:28
作者
Anik, Ulku [1 ]
Odaci, Dilek
Timur, Suna
Merkoci, Arben
Pazarlioglu, Nurdan
Telefoncu, Azmi
Alegret, Salvador
机构
[1] Mugla Univ, Fac Arts & Sci, Dept Chem, Kotekli, Turkey
[2] Ege Univ, Fac Sci, Dept Biochem, TR-35100 Bornova, Turkey
[3] Univ Autonoma Barcelona, Dept Quim, Grp Sensors & Biosensors, Bellaterra 08193, Catalonia, Spain
关键词
microbial biosensor; graphite-epoxy composite electrode; Pseudomonas putida; RIGID CARBON; BIOCOMPOSITE; SURFACE;
D O I
10.1016/j.bioelechem.2005.11.002
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The modification of a graphite-epoxy composite electrode (GECE) with bacterial cells along with an analytical application are presented. Pseudomonas putida DSM 50026 was used as a biological component and the measurement was based on the respiratory activity of the cells. The optimization of working conditions of resulting biosensor (including pH and temperature) was conducted and the limit of detection was calculated as 7 mu M phenol based on the signal to noise ratio. Then the system was applied for xenobiotic detection. Resulting sample signals were found to be very similar with the standard solutions having the same concentration while the recoveries of the spiked samples were close to 100%. (c) 2006 Published by Elsevier B.V.
引用
收藏
页码:128 / 131
页数:4
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