Identification of a human influenza type B strain with reduced sensitivity to neuraminidase inhibitor drugs

被引:21
作者
Hurt, AC
McKimm-Breschkin, JL
McDonald, M
Barr, IG
Komadina, N
Hampson, AW
机构
[1] WHO Collaborating Ctr Reference & Res Influenza, Melbourne, Vic 3052, Australia
[2] CSIRO, Div Hlth Sci & Nutr, Melbourne, Vic, Australia
关键词
influenza; neuraminidase inhibitors; reduced sensitivity; oseltamivir carboxylate; zanamivir; RWJ-270201; chemiluminescent; fluorescent;
D O I
10.1016/j.virusres.2004.02.035
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A total of 126 influenza B isolates isolated between 1998 and 2002 from Australasia and the Asia-Pacific region were tested for their sensitivity to the neuraminidase (NA) inhibitor drugs zanamivir and oseltamivir carboxylate using a fluorescence-based enzyme assay. The mean (+/-1 S.D.) 50% inhibitory concentration (IC50) of the influenza B viruses tested was 1.41 +/- 0.53 nM against zanamivir and 14.91 +/- 14.31 nM with oseltamivir carboxylate. However, a single type B isolate (B/Perth/211/2001) from an infant who had not been treated with either of the NA inhibitor drugs, showed a nine-fold lower sensitivity to zanamivir and a 14-fold lower sensitivity to oseltamivir carboxylate compared with the mean IC50 of influenza B strains. A decrease in sensitivity to oseltamivir carboxylate and RWJ-270201 was also seen in both: a chemiluminescent assay and a second different fluorescent assay. Sequence analysis of the haemagglutinin HA I region and the neuraminidase gene of B/Perth/211/2001 revealed no amino acid changes in sites that have previously been reported to confer resistance to either of the NAI drugs. Further investigations are in progress to identify the basis for this reduced sensitivity. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:205 / 211
页数:7
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