Piezoelectric crystal for sensing bacteria by immobilizing antibodies on divinylsulphone activated poly-m-aminophenol film

被引：26

作者：

Fung, YS ^{[1
]}

Si, SH ^{[1
]}

Zhu, DR ^{[1
]}

机构：

[1] Univ Hong Kong, Dept Chem, Hong Kong, Peoples R China

来源：

TALANTA | 2000年 / 51卷 / 01期

关键词：

piezoelectric crystal; bacteria; antibodies; divinylsulphone; poly-m-aminophenol film;

D O I：

10.1016/S0039-9140(99)00266-0

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A polymer sorbent formed at the surface of gold-plated piezoelectric crystal by anodic oxidation of m-aminophenol was investigated by voltammetric, FTIR techniques for antibody coupling after activation by divinylsulphone. A novel film with increased capacity for immobilizing antibodies was obtained using phloroglucinol to modify poly-m-aminophenol via divinylsulphone. Compared with the dip-coating methods using polyethylenimine and (gamma-aminopropyl)trimethoxysilane, this new technique gave more reproducible results for the immobilization of antibody from sample to sample due to the improved homogeneity and reproducibility of the coating. With present modification method, a piezoelectric immunosensor was developed for the detection of Salmonella enteritidis. A detection limit of 1 x 10(5) cells ml(-1) and an assay time of 25 min were achieved. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：151 / 158

页数：8

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