Identification of genes regulating colorectal carcinogenesis by using the algorithm for diagnosing malignant state method

被引：16

作者：

Ichikawa, Y

Ishikawa, T

Takahashi, S

Hamaguchi, Y

Morita, T

Nishizuka, I

Yamaguchi, S

Endo, I

Ike, H

Togo, S

Oki, S

Shimada, H ^{[1
]}

Kadota, K

Nakamura, S

Goto, H

Nitanda, H

Satomi, S

Sakai, T

Narita, I

Gejyo, F

Tomaru, Y

Shimizu, K

Hayashizaki, Y

Okazaki, Y

机构：

[1] Yokohama City Univ, Sch Med, Dept Surg 2, Yokohama, Kanagawa, Japan

[2] RIKEN, Yokohama Inst, Genom Sci Ctr, Lab Genome Explorat Res Grp,Tsurumi Ku, Yokohama, Kanagawa 2300045, Japan

[3] Univ Tokyo, Dept Biotechnol, Tokyo, Japan

[4] Tohoku Univ, Grad Sch Med, Dept Adv Surg Sci & Technol, Sendai, Miyagi, Japan

[5] Niigata Univ, Grad Sch Med & Dent Sci, Div Clin Nephrol & Rheumatol, Ibaraki, Japan

[6] Univ Tsukuba, Inst Basic Med Sci & Biol Sci, Tsukuba, Ibaraki 305, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2002年 / 296卷 / 02期

关键词：

gene expression profiling; molecular classification; colorectal cancer; malignancy diagnosis; DNA microarray;

D O I：

10.1016/S0006-291X(02)00732-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

We studied the expression profiles of various stages of colorectal tumors (adenoma (AD), seven samples; carcinoma (CA), 16 samples) by using cDNA microarrays and developed ADMS (algorithm for diagnosing malignant state) method, selecting 335 clones characteristic of CA state. We, then, applied ADMS to 12 additional samples (five from primary lesions with metastasis and seven metastases); all 16 CAs and 12 metastatic tumors were diagnosed correctly as cancerous states, Although three of the seven ADs were diagnosed as "'cancerous," the large size of two of these tumors suggested their potential malignancy. Our strategy for selecting clones characteristic of the malignant state is widely applicable to diagnosis and for predicting the stage of progression during multistep carcinogenesis. Of the 335 clones we selected, 135 were known genes. Included in the 135 genes were tumor suppressor and growth factor-related genes and were consistent with the literature. ADMS is a reliable means for identifying genes useful for the diagnosis of cancer. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：497 / 506

页数：10

共 21 条

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CHROMOSOME-17 DELETIONS AND P53 GENE-MUTATIONS IN COLORECTAL CARCINOMAS [J].