Strain differences in binding properties of estrogen receptors in immature and adult BALB/c and MRL/MP-lpr/lpr mice, a model of systemic lupus erythematosus

被引:17
作者
Dhaher, YY
Greenstein, B [1 ]
Nunn, ED
Khamashta, M
Hughes, GRV
机构
[1] Univ Arizona, Arizona Arthrit Ctr, 1501 N Campbell Ave,Box 245093, Tucson, AZ 85724 USA
[2] Schiller Int Univ, London SE1, England
[3] St Thomas Hosp, Rayne Inst, Lupus Res Unit, London SE1 7EH, England
[4] Medicare, Ramallah, Palestine
来源
INTERNATIONAL JOURNAL OF IMMUNOPHARMACOLOGY | 2000年 / 22卷 / 03期
关键词
D O I
10.1016/S0192-0561(99)00090-9
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The aim was to compare binding properties of estrogen receptors in brain, reproductive and immune tissues of immature and adult female BALB/c mice, and in the same tissues of MRL/MP-lpr/lpr mice. The latter strain spontaneously develops an autoimmune disease resembling human systemic lupus erythematosus (lupus; SLE). It is hypothesized that estradiol, through its receptors, mediates the progression of murine SLE. High-speed cytosols were prepared from hypothalamus, spleen, thymus and uterus of both strains, and incubated with the synthetic estrogen H-3-moxestrol (NEN). Scatchard plots were derived from binding isotherms obtained after in vitro incubation. In addition, cervical lymph nodes from MRL mice could be used, but were too small in BALB/c mice. There was a significant increase in the affinity of the binding reaction i.e. a decrease in the apparent molar dissociation constant (Kd), in immune tissues and uterus with maturation in MRL but not BALB/c mice, whose tissues had, overall, a lower affinity for H-3-moxestrol. Receptor concentrations were significantly higher in spleen and cervical lymph nodes of adult compared with immature MRL mice, but the opposite pattern was observed in BALB/c mouse spleen on maturation. These properties of estrogen receptors in MRL mice may underlie estrogen-mediated exacerbation of murine SLE. (C) 2000 International Society for Immunopharmacology. Published by Elsevier Science Ltd. All rights reserved.
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页码:247 / 254
页数:8
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