Reversible skeletal neuromuscular paralysis induced by different lysophospholipids

被引：27

作者：

Caccin, Paola ^{[1
]}

Rigoni, Michela ^{[1
]}

Bisceglie, Alessandra ^{[1
]}

Rossetto, Ornella ^{[1
]}

Montecucco, Cesare ^{[1
]}

机构：

[1] Univ Padua, Ist Neurosci, CNR, Dipartimento Sci Biomed, I-35121 Padua, Italy

来源：

FEBS LETTERS | 2006年 / 580卷 / 27期

关键词：

neuromuscular junction; paralysis; snake neurotoxins; phospholipase A2; lysophospholipids;

D O I：

10.1016/j.febslet.2006.10.039

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lysophosphatidylcholine rapidly paralyses the neuromuscular junction (NMJ), similarly to snake phospholipase A2 neurotoxins, implicating a lipid hemifusion-pore transition in neuroexocytosis. The mode and kinetics of NMJ paralysis of different lysophospholipids (lysoPLs) in high or low [Mg2+] was investigated. The following order of potency was found: lysophosphatidylcholine > lysophosphatidylethanolamine > lysophosphatidic acid > lysophosphatidylserine > lysophosphatidylglycerol. The latter two lysoPLs closely mimic the profile of paralysis caused by the toxins in high [Mg2+]. This paralysis is fully reversed by albumin washing. These findings provide novel insights on the mode of action of snake neurotoxins and qualify lysoPLs as novel agents to study neuroexocytosis. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：6317 / 6321

页数：5

共 25 条

[1] Taipoxin induces synaptic vesicle exocytosis and disrupts the interaction of synaptophysin I with VAMP2 [J].

Bonanomi, D ;

Pennuto, M ;

Rigoni, M ;

Rossetto, O ;

Montecucco, C ;

Valtorta, F .

MOLECULAR PHARMACOLOGY, 2005, 67 (06) :1901-1908

[2]

BULBRING E, 1946, BRIT J PHARM CHEMOTH, V1, P38

[3]

CHEN I, 1970, VIRCHOWS ARCH B, V6, P318

[4] Protein-lipid interplay in fusion and fission of biological membranes [J].

Chernomordik, LV ;

Kozlov, MM .

ANNUAL REVIEW OF BIOCHEMISTRY, 2003, 72 :175-207

[5] EFFECTS OF TAIPOXIN AND NOTEXIN ON FUNCTION AND FINE-STRUCTURE OF MURINE NEUROMUSCULAR-JUNCTION [J].

CULLCANDY, SG ;

FOHLMAN, J ;

GUSTAVSSON, D ;

LULLMANNRAUCH, R ;

THESLEFF, S .

NEUROSCIENCE, 1976, 1 (03) :175-&

[6] Crystal structure of human serum albumin complexed with fatty acid reveals an asymmetric distribution of binding sites [J].

Curry, S ;

Mandelkow, H ;

Brick, P ;

Franks, N .

NATURE STRUCTURAL BIOLOGY, 1998, 5 (09) :827-835

[7] Nerve terminal damage by β-bungarotoxin -: Its clinical significance [J].

Dixon, RW ;

Harris, JB .

AMERICAN JOURNAL OF PATHOLOGY, 1999, 154 (02) :447-455

[8] MORPHOLOGICAL-CHANGES INDUCED BY CROTOXIN IN MURINE NERVE AND NEUROMUSCULAR-JUNCTION [J].

GOPALAKRISHNAKONE, P ;

HAWGOOD, BJ .

TOXICON, 1984, 22 (05) :791-804

[9] The neurotoxicity of the venom phospholipases A2, notexin and taipoxin [J].

Harris, JB ;

Grubb, BD ;

Maltin, CA ;

Dixon, R .

EXPERIMENTAL NEUROLOGY, 2000, 161 (02) :517-526

[10]

Harris John B., 1996, Symposia of the Zoological Society of London, V70, P235

← 1 2 3 →