Induced angiogenesis under cerebral ischemia by cyclooxygenase 2 and hypoxia-inducible factor naked DNA in a rat indirect-bypass model

被引：28

作者：

Anan, Mitsuhiro ^{[1
]}

Abe, Tatsuya ^{[1
]}

Matsuda, Takeshi ^{[1
]}

Ishii, Keisuke ^{[1
]}

Kamida, Tohru ^{[1
]}

Fujiki, Minoru ^{[1
]}

Kobayashi, Hidenori ^{[1
]}

机构：

[1] Oita Univ, Dept Neurosurg, Sch Med, Oita 8795593, Japan

来源：

NEUROSCIENCE LETTERS | 2006年 / 409卷 / 02期

关键词：

HIF; COX-2; angiogenesis; ischemia; encephalo-myo-synangosis;

D O I：

10.1016/j.neulet.2006.09.039

中图分类号：

Q189 [神经科学];

学科分类号：

071006 [神经生物学];

摘要：

We recently reported that hypoxic stress induces the expression of HIF-1 alpha, HIF-2 alpha, cyclooxygenases-2 (COX-2) and VEGF in vivo. In this study, we investigated whether HIF-1 alpha, HIF-2 alpha, or COX-2 naked DNA induced angiogenesis in a cerebral ischemic model in vivo. We utilized a rat encephalo-myo-synangiosis (EMS) model and inoculated naked DNA into the brain surface. We analyzed whether DNA induced angiogenic factors and neovascularization. New blood vessel formation was detected by anti-Factor VIII staining. A histological section treated with HIF-2 alpha or COX-2 DNA showed an increased expression of VEGF with angiogenesis, in comparison to the control DNA. The HIF-1 alpha, HIF-2 alpha, and COX-2 are able to promote significant angiogenesis development. These results suggest the feasibility of a novel approach for therapeutic angiogenesis of cerebral ischemia in which neovascularization may be indirectly achieved using a transcriptional and cytokine's regulatory strategy. (c) 2006 Elsevier Ireland Ltd. All rights reserved.

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收藏

页码：118 / 123

页数：6

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