Novel approach to control Salmonella enterica by modern biophotonic technology: photosensitization

被引:38
作者
Buchovec, I. [1 ]
Vaitonis, Z. [1 ]
Luksiene, Z. [1 ]
机构
[1] Vilnius State Univ, Inst Mat Sci & Appl Res, LT-10223 Vilnius, Lithuania
关键词
5-aminolevulinic acid; decontamination technology; pathogen control; photosensitization; Salmonella; GRAM-NEGATIVE BACTERIA; PROTOPORPHYRIN-IX; INACTIVATION;
D O I
10.1111/j.1365-2672.2008.03993.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Salmonellosis is one of the most common foodborne diseases in the world. The aim of this study was to evaluate the antibacterial efficiency of 5-aminolevulinic acid (ALA) based photosensitization against one of food pathogens Salmonella enterica. Salmonella enterica was incubated with ALA (7.5 mmol l(-1)) for 1-4 h and afterwards illuminated with visible light. The light source used for illumination of S. enterica emitted light lambda = 400 nm with energy density 20 mW cm(-2). The illumination time varied from 0 to 20 min and subsequently a total energy dose reached 0-24 J cm(-2). The data obtained indicate that S. enterica is able to produce endogenous photosensitizer PpIX when incubated with ALA. Remarkable inactivation of micro-organisms can be achieved (6 log) after photosensitization. It is obvious that photosensitization-based inactivation of S. enterica depends on illumination as well as incubation with ALA time. ALA-based photosensitization can be an effective tool against multi-drug resistant Gram-negative bacteria S. enterica serovar Typhimurium. Experimental data and mathematical evaluations support the idea that ALA-based photosensitization can be a useful tool for the development of nonthermal food preservation technology in future.
引用
收藏
页码:748 / 754
页数:7
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