Gibberellin Modulates Anther Development in Rice via the Transcriptional Regulation of GAMYB

被引:328
作者
Aya, Koichiro [1 ,2 ]
Ueguchi-Tanaka, Miyako [1 ]
Kondo, Maki [3 ]
Hamada, Kazuki [4 ]
Yano, Kentaro [4 ]
Nishimura, Mikio [3 ]
Matsuoka, Makoto [1 ]
机构
[1] Nagoya Univ, Biosci & Biotechnol Ctr, Nagoya, Aichi 4648601, Japan
[2] Japan Soc Promot Sci, Chiyoda Ku, Tokyo 1028472, Japan
[3] Natl Inst Basic Biol, Dept Cell Biol, Okazaki, Aichi 4448585, Japan
[4] Meiji Univ, Fac Agr, Kawasaki, Kanagawa 2418571, Japan
关键词
ARABIDOPSIS-THALIANA; DELLA PROTEIN; FLORAL DEVELOPMENT; SIGNALING PATHWAY; ALEURONE CELLS; GENE ENCODES; POLLEN; RECEPTOR; MUTANT; SPOROPOLLENIN;
D O I
10.1105/tpc.108.062935
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Gibberellins (GAs) play important roles in regulating reproductive development, especially anther development. Our previous studies revealed that the MYB transcriptional factor GAMYB, an important component of GA signaling in cereal aleurone cells, is also important for anther development. Here, we examined the physiological functions of GA during anther development through phenotypic analyses of rice (Oryza sativa) GA-deficient, GA-insensitive, and gamyb mutants. The mutants exhibited common defects in programmed cell death (PCD) of tapetal cells and formation of exine and Ubisch bodies. Microarray analysis using anther RNAs of these mutants revealed that rice GAMYB is involved in almost all instances of GA-regulated gene expression in anthers. Among the GA-regulated genes, we focused on two lipid metabolic genes, a cytochrome P450 hydroxylase CYP703A3 and beta-ketoacyl reductase, both of which might be involved in providing a substrate for exine and Ubisch body. GAMYB specifically interacted with GAMYB binding motifs in the promoter regions in vitro, and mutation of these motifs in promoter-beta-glucuronidase (GUS) transformants caused reduced GUS expression in anthers. Furthermore, a knockout mutant for CYP703A3 showed gamyb-like defects in exine and Ubisch body formation. Together, these results suggest that GA regulates exine formation and the PCD of tapetal cells and that direct activation of CYP703A3 by GAMYB is key to exine formation.
引用
收藏
页码:1453 / 1472
页数:20
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