Conductive wiring of immobilized photosynthetic reaction center to electrode by cytochrome c

被引:100
作者
Lebedev, Nikolai [1 ]
Trammell, Scott A.
Spano, Anthony
Lukashev, Evgeny
Griva, Igor
Schnur, Joel
机构
[1] USN, Res Lab, Ctr Biomol Sci & Engn, Washington, DC 20375 USA
[2] Univ Virginia, Dept Biol, Charlottesville, VA 22904 USA
[3] George Mason Univ, Dept Math Sci, Fairfax, VA 22030 USA
[4] George Mason Univ, Dept Computat Sci, Fairfax, VA 22030 USA
关键词
D O I
10.1021/ja063367y
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The photosynthetic reaction center (RC) found in photosynthetic bacteria is one of the most advanced photoelectronic devices developed by nature. However, after immobilization on the electrode surface, the efficiency of electron transfer (ET) between the RC and the electrode is relatively low. This inefficiency has limited the possibility of using the RC for technological applications. Here we show that photoinduced electron transfer between the immobilized RC and a gold electrode can be increased by several tens-fold by incorporation of cytochrome c into the RC-self-assembled monolayer (SAM)-electrode complex. The effect does not depend on the initial redox state of the cytochrome and seems to be the result of the formation of a complex between the RC and the cytochrome c serving as an ET wire. This observation opens the possibility for electrochemical analysis of the special pair in the RC protein that is deeply buried inside the protein globe and is barely electrically addressable from the electrode surface.
引用
收藏
页码:12044 / 12045
页数:2
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