Cu(II)-incorporated histidine-containing, magnetic-metal-complexing beads as specific sorbents for the metal chelate affinity of albumin

被引:50
作者
Akgöl, S [1 ]
Türkmen, D [1 ]
Denizli, A [1 ]
机构
[1] Hacettepe Univ, Dept Chem, Div Biochem, Ankara, Turkey
关键词
magnetic polymers; seperation techniques; proteins; tyrosine;
D O I
10.1002/app.20757
中图分类号
O63 [高分子化学(高聚物)];
学科分类号
070305 ; 080501 ; 081704 ;
摘要
N-Methacryloyl-(L)-histidine methyl ester (MAH) was synthesized from metharyloyl chloride and histidine. Spherical beads with an average size of 150-250 mum were obtained by the suspension polymerization of ethylene glycol dimethacrylate and MAH in an aqueous dispersion medium. Magnetic poly(ethylene glycol dimethacrylate-co-N-Methacryloyl-(L)-histidine methyl ester) [m-p(EGDMA-co-MAH)] microbeads were characterized with swelling tests, electron spin resonance, elemental analysis, and scanning electron microscopy. The specific surface area of the beads was 80.1 m(2)/g. m-p(EGDMA-co-MAH) microbeads with a swelling ratio of 40.2% and 43.9 mumol of MAH/g were used for the adsorption of bovine serum albumin (BSA) in a batch system. The Cu(II) concentration was 4.1 mumol/g. The adsorption capacity of BSA on the Cu(II)-incorporated beads was 19.2 mg of BSA/g. The BSA adsorption first increased with the BSA concentration and then reached a plateau, which was about 19.2 mg of BSA/g. The maximum adsorption was observed at pH 5.0, which was the isoelectric point of BSA. The BSA adsorption increased with decreasing temperature, and the maximum adsorption was achieved at 4degreesC. High desorption ratios (>90% of the adsorbed BSA) were achieved with 1.0M NaSCN (pH 8.0) in 30 min. The nonspecific adsorption of BSA onto the mp(EGDMA-co-MAH) beads was negligible. (C) 2004 Wiley Periodicals, Inc.
引用
收藏
页码:2669 / 2677
页数:9
相关论文
共 25 条
[1]   Reversible immobilization of urease onto Procion Brown MX-5BR-Ni(II) attached polyamide hollow-fibre membranes [J].
Akgöl, S ;
Yalçinkaya, Y ;
Bayramoglu, G ;
Denizli, A ;
Arica, MY .
PROCESS BIOCHEMISTRY, 2002, 38 (05) :675-683
[2]   Hydrolysis of sucrose by invertase immobilized onto novel magnetic polyvinylalcohol microspheres [J].
Akgöl, S ;
Kaçar, Y ;
Denizli, A ;
Arica, MY .
FOOD CHEMISTRY, 2001, 74 (03) :281-288
[3]   METAL-AFFINITY SEPARATIONS - A NEW DIMENSION IN PROTEIN PROCESSING [J].
ARNOLD, FH .
BIO-TECHNOLOGY, 1991, 9 (02) :151-156
[4]   Performance of immobilized glucoamylase in a magnetically stabilized fluidized bed reactor (MSFBR) [J].
Bahar, T ;
Çelebi, SS .
ENZYME AND MICROBIAL TECHNOLOGY, 2000, 26 (01) :28-33
[5]  
BAUMBACH GA, 1992, BIOPHARM-TECHNOL BUS, V5, P24
[6]   Structural damage to lactate dehydrogenase during copper iminodiacetic acid metal affinity chromatography [J].
Bush, KD ;
Lumpkin, JA .
BIOTECHNOLOGY PROGRESS, 1998, 14 (06) :943-950
[7]   PREPARATION AND PROPERTIES OF SERUM AND PLASMA PROTEINS .4. A SYSTEM FOR THE SEPARATION INTO FRACTIONS OF THE PROTEIN AND LIPOPROTEIN COMPONENTS OF BIOLOGICAL TISSUES AND FLUIDS [J].
COHN, EJ ;
STRONG, LE ;
HUGHES, WL ;
MULFORD, DJ ;
ASHWORTH, JN ;
MELIN, M ;
TAYLOR, HL .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1946, 68 (03) :459-475
[8]  
Denizli A, 1997, J APPL POLYM SCI, V65, P2085, DOI 10.1002/(SICI)1097-4628(19970912)65:11<2085::AID-APP4>3.0.CO
[9]  
2-D
[10]   ATOMIC-STRUCTURE AND CHEMISTRY OF HUMAN SERUM-ALBUMIN [J].
HE, XM ;
CARTER, DC .
NATURE, 1992, 358 (6383) :209-215