Antiallergic tea catechin, (-)-epigallocatechin-3-O-(3-O-methyl)-gallate, suppresses FcεRI expression in human basophilic KU812 cells

被引:92
作者
Fujimura, Y
Tachibana, H
Maeda-Yamamoto, M
Miyase, T
Sano, M
Yamada, K
机构
[1] Kyushu Univ, Fac Agr, Dept Biosci & Biotechnol, Higashi Ku, Fukuoka 8128581, Japan
[2] Natl Res Inst Vegetables & Tea Sci, Shizuoka 4288501, Japan
[3] Univ Shizuoka, Sch Pharmaceut Sci, Shizuoka 4228526, Japan
关键词
tea catechin; basophil; KU812; Fc epsilon RI; O-methylated EGCg;
D O I
10.1021/jf025680z
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
We previously found that the O-methylated derivative of (-) epigallocatechin 3 O-gallate (EGCg), (-)-epigallocatechin 3 O-(3-O-methyl)-gallate (EGCG"3Me) has potent antiallergic activity The high-affinity IgE receptor, FcepsilonRI, is found at high levels on basophils and mast cells and plays a key role in a series of acute and chronic human allergic reactions To understand the mechanism of action for the antiallergic EGCG"3Me, the effect of EGCG"3Me on the cell surface expression of FcepsilonRI in human basophilic KU812 cells was examined Flow cytometric analysis showed that EGCG"3Me was able to decrease the cell surface expression of FcepsilonRI Moreover, immunoblot analysis revealed that total cellular expression of the FcepsilonRI alpha chain decreased upon treatment with EGCG"3Me FcepsilonRI is a tetrameric structure comprising one alpha chain, one beta chain, and two gamma chains The level of mRNA production of each subunit in KU812 cells was investigated EGCG"3Me reduced FcepsilonRI alpha and gamma mRNA levels The cross-linkage of FcepsilonRI causes the activation of basophils which leads to the secretion of inflammatory mediators including histamine EGCG"3Me treatment inhibited the FcepsilonRI cross-linking induced histamine release These results suggested that EGCG"3Me can negatively regulate basophil activation through the suppression of FcepsilonRI expression.
引用
收藏
页码:5729 / 5734
页数:6
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