Spatial distribution of single-photon and two-photon fluorescence light in scattering media: Monte Carlo simulation

被引：14

作者：

Gan, XS ^{[1
]}

Gu, M ^{[1
]}

机构：

[1] Victoria Univ Technol, Sch Commun & Informat, Optoelect Imaging Grp, Melbourne, Vic 8001, Australia

来源：

APPLIED OPTICS | 2000年 / 39卷 / 10期

关键词：

D O I：

10.1364/AO.39.001575

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Three-dimensional fluorescence spatial distributions under single-photon and two-photon excitation within a turbid medium are studied with Monte Carlo simulation. It is demonstrated that two-photon excitation has an advantage of producing much less fluorescence light outside the focal region compared with single-photon excitation. With the increase of the concentration of scattering particles in a turbid medium, the position of the maximum fluorescence intensity point shifts from the geometric focal region toward the medium surface. Further studies show that the optical sectioning property of two-photon fluorescence microscopy is degraded in thick turbid media or when the numerical aperture of an objective becomes low. (C) 2000 Optical Society of America.

引用

页码：1575 / 1579

页数：5

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