Synergetic effect for NADH oxidation of ferrocene and zeolite in modified carbon paste electrodes - New approach for dehydrogenase based biosensors

被引:46
作者
Serban, S [1 ]
El Murr, N [1 ]
机构
[1] Univ Nantes, Fac Sci & Tech, Lab Anal Isotop & Electrochim Metab, CNRS,UMR 6006, F-44322 Nantes 03, France
关键词
NADH oxidation; ferrocene; zeolite; carbon paste electrode; modified electrode;
D O I
10.1016/j.bios.2004.01.030
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
The development of electrochemical biosensors using dehydrogenases associated with the corresponding cofactor is strongly related to the better understanding of NADH oxidation at the electrode surface. The aim is to lower the necessary overvoltage and consequently to escape interferences and electrode fouling. In this paper, we show that carbon paste electrode (CPE) modified with NaY zeolite fulfils this requirements thanks to its hydrophilic surface. Oxidation of NADH at ferrocene (FcH) modified carbon paste electrode exhibits a rather slow electrocatalytic effect. We demonstrated the existence of synergetic effect on the electrocatalytic oxidation of NADH when the CPE is doped with zeolite (NaY) and FcH mediator or with the zeolite exchanged beforehand with the mediator (Y-Ferricinium, YFcH). This cumulative effect permits to reach high sensitivity for NADH detection and offers new way for the development of enzymatic biosensors using dehydrogenases depending on NADH as cofactor. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:161 / 166
页数:6
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