Comparative Analyses of Disease Risk Genes Belonging to the Acyl-CoA Synthetase Medium-Chain (ACSM) Family in Human Liver and Cell Lines

被引：40

作者：

Boomgaarden, Inka ^{[1
]}

Vock, Christina ^{[1
]}

Klapper, Maja ^{[1
]}

Doering, Frank ^{[1
]}

机构：

[1] Univ Kiel, Dept Mol Prevent, Inst Human Nutr & Food Sci, D-24118 Kiel, Germany

来源：

BIOCHEMICAL GENETICS | 2009年 / 47卷 / 9-10期

关键词：

Acyl-CoA synthetase medium-chain (ACSM); Medium-chain fatty acid; Liver; HuH-7; HepG2; HUMAN HEPATOCYTES; FATTY-ACIDS; SAH; HYPERTENSION; EXPRESSION; CAUCASIANS; VARIANTS; SEQUENCE; HORMONE; OBESITY;

D O I：

10.1007/s10528-009-9273-z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The human ACSM1, 2A and B, 3, and 5 genes, located on chromosome 16p12-13, encode for enzymes catalyzing the activation of medium-chain length fatty acids. Association studies have linked several polymorphisms of these genes to traits of insulin resistance syndrome. In our study, ACSM transcripts showed 3 to > 400-fold higher expression levels in human liver when compared to cell lines by qRT-PCR. This difference was also evident at the protein level, as shown for ACSM2. In liver, ACSM2 was the most abundant transcript, showing sixfold (vs. ACSM3) to > 300-fold higher expression levels (vs. ACSM1). Mitochondrial localization of the ACSM2 protein and the presence of an N-terminal targeting sequence were shown by GFP-tagging. We have shown ACSM2B to be the predominant transcript in human liver, and genetic variations of this gene could therefore play an important role in disease susceptibility.

引用

页码：739 / 748

页数：10

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