Reversal of synaptic vesicle docking at central synapses

被引：187

作者：

Murthy, VN

Stevens, CF

机构：

[1] Salk Inst Biol Studies, Howard Hughes Med Inst, La Jolla, CA 92037 USA

[2] Salk Inst Biol Studies, Mol Neurobiol Lab, La Jolla, CA 92037 USA

来源：

NATURE NEUROSCIENCE | 1999年 / 2卷 / 06期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1038/9149

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We used quantitative fluorescence imaging of vesicles labeled with membrane-soluble dyes to determine rates of undocking and spontaneous exocytosis of vesicles docked to the active zone of hippocampal synapses in culture. Individual vesicles undock about once per two minutes and spontaneously exocytose about once per eight minutes. Thus, not only does undocking occur, but it is over threefold faster than spontaneous fusion.

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页码：503 / 507

页数：5

相关论文

共 23 条

[1] OPTICAL ANALYSIS OF SYNAPTIC VESICLE RECYCLING AT THE FROG NEUROMUSCULAR-JUNCTION [J].

BETZ, WJ ;

BEWICK, GS .

SCIENCE, 1992, 255 (5041) :200-203

[2] Neuronal peptide release is limited by secretory granule mobility [J].

Burke, NV ;

Han, WP ;

Li, DQ ;

Takimoto, K ;

Watkins, SC ;

Levitan, ES .

NEURON, 1997, 19 (05) :1095-1102

[3]

Calakos N, 1996, PHYSIOL REV, V76, P1

[4]

Fesce Riccardo, 1994, Trends in Cell Biology, V4, P1, DOI 10.1016/0962-8924(94)90025-6

[5] SYNAPTOTAGMIN-I - A MAJOR CA2+ SENSOR FOR TRANSMITTER RELEASE AT A CENTRAL SYNAPSE [J].

GEPPERT, M ;

GODA, Y ;

HAMMER, RE ;

LI, C ;

ROSAHL, TW ;

STEVENS, CF ;

SUDHOF, TC .

CELL, 1994, 79 (04) :717-727

[6] 2 COMPONENTS OF TRANSMITTER RELEASE AT A CENTRAL SYNAPSE [J].

GODA, Y ;

STEVENS, CF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (26) :12942-12946

[7] Two distinct pools of synaptic vesicles in single presynaptic boutons in a temperature-sensitive Drosophila mutant, shibire [J].

Kuromi, H ;

Kidokoro, Y .

NEURON, 1998, 20 (05) :917-925

[8] Stages of regulated exocytosis [J].

Martin, TFJ .

TRENDS IN CELL BIOLOGY, 1997, 7 (07) :271-276

[9] Synaptic vesicles retain their identity through the endocytic cycle [J].

Murthy, VN ;

Stevens, CF .

NATURE, 1998, 392 (6675) :497-501

[10] Heterogeneous release properties of visualized individual hippocampal synapses [J].

Murthy, VN ;

Sejnowski, TJ ;

Stevens, CF .

NEURON, 1997, 18 (04) :599-612