Development of a broadly reactive nested reverse transcription-PCR assay to detect murine noroviruses, and investigation of the prevalence of murine noroviruses in laboratory mice in Japan

被引：26

作者：

Kitajima, Masaaki ^{[1
]}

Oka, Tomoichiro ^{[2
]}

Tohya, Yukinobu ^{[3
]}

Katayama, Hiroyuki ^{[1
]}

Takeda, Naokazu ^{[2
]}

Katayama, Kazuhiko ^{[2
]}

机构：

[1] Univ Tokyo, Grad Sch Engn, Dept Urban Engn,Bunkyo Ku, Publ Hlth Lab, Tokyo 1138656, Japan

[2] Natl Inst Infect Dis, Dept Virol 2, Tokyo 2080011, Japan

[3] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Vet Microbiol, Bunkyo Ku, Tokyo 1138657, Japan

来源：

MICROBIOLOGY AND IMMUNOLOGY | 2009年 / 53卷 / 09期

关键词：

laboratory mice; murine noroviruses; nested RT-PCR; phylogenetic analysis; MURINE-NOROVIRUS-1; INFECTION; MOLECULAR CHARACTERIZATION; GENOGROUP; DIVERSITY;

D O I：

10.1111/j.1348-0421.2009.00152.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A broadly reactive nested RT-PCR assay to detect MNV was developed and subsequently used to investigate the prevalence of MNV in laboratory mice in Japan. MNV were detected in 8 (22%) of 37 murine stool specimens by second-round PCR, although no positive band was obtained from any specimen by first-round PCR. Genetic analysis of the second round PCR products showed that MNV sequences detected in this study were closely matched (97.2 similar to 99.1%) to that of MNV-3 (DQ223042). This is the first report demonstrating the prevalence of MNV in Japan.

引用

页码：531 / 534

页数：4

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