Ultrafast gain dynamics of the green fluorescent protein

被引：9

作者：

Didier, P

Guidoni, L

Schwalbach, G

Bourotte, M

Follenius-Wund, A

Pigault, C

Bigot, JY

机构：

[1] ULP, CNRS, UMR 7504, Inst Phys & Chim Mat Strasbourg, F-67037 Strasbourg, France

[2] ULP Biotechnol Interact Macromol, CNRS, UMR 7100, F-67400 Illkirch Graffenstaden, France

[3] ULP, Lab Pharmacochim Commun Cellulaire, CNRS, UMR 7081, F-67400 Illkirch Graffenstaden, France

[4] ULP Pharmacol & Physicochim Interact Cellulaires, CNRS, UMR 7034, F-67400 Illkirch Graffenstaden, France

来源：

CHEMICAL PHYSICS LETTERS | 2002年 / 364卷 / 5-6期

关键词：

D O I：

10.1016/S0009-2614(02)01379-9

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

We investigated the excited state dynamics of the uv mutant of the green fluorescent protein (GFPuv) using spectrally resolved femtosecond pump-probe spectroscopy. The gain dynamics of GFPuv is characterized by a 'slow' picosecond mono-exponential growth contrary to the wild type (GFPwt) where ultrafast components have been previously reported. As GFPwt and GFPuv share the same chromophore, this difference can be attributed to an intrinsic improved homogeneity of the GFPuv sample. The study of a synthetic GFP chromophore allows us to confirm the key-role played by the interactions chromophore-proteic cage in GFP photophysics. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：503 / 510

页数：8

共 25 条

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