Formation of mediated biocatalytic cathodes by electrodeposition of a redox polymer and laccase

被引：15

作者：

Hudak, Nicholas S. ^{[1
]}

Gallaway, Joshua W. ^{[1
]}

Barton, Scott Calabrese ^{[2
]}

机构：

[1] Columbia Univ, Dept Chem Engn, New York, NY 10027 USA

[2] Michigan State Univ, Dept Chem Engn & Mat Sci, E Lansing, MI 48824 USA

来源：

JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 2009年 / 629卷 / 1-2期

关键词：

Laccase; Redox polymer; Electrodeposition; Mediator; Enzyme electrode; Bioelectrocatalysis; GLUCOSE-OXIDASE; BIOFUEL CELL; AMPEROMETRIC DETERMINATION; CARBON ELECTRODES; LIGAND-EXCHANGE; ENZYME; ELECTROCHEMISTRY; BIOSENSOR; IMMOBILIZATION; PURIFICATION;

D O I：

10.1016/j.jelechem.2009.01.021

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Mediated biocatalytic cathodes were fabricated via electrodeposition of an osmium-based redox polymer onto planar and porous electrode surfaces, incorporating the oxygen-reducing enzyme laccase. After one hour of electrodeposition time, bio-cathodes on glassy carbon substrate exhibited redox site loading of 6 nmol cm(-2) and oxygen reduction current density of 1.4 mA cm-2. Those on carbon-fiber paper exhibited redox site loading of 46 nmol cm(-2) and oxygen reduction current density of 15.7 mA cm(-2). The current density and polarization behavior were comparable to those of bio-cathodes made by conventional chemical crosslinking. The specific activity in terms of current per mole of redox polymer or enzyme was significantly higher for electrodeposited films. (c) 2009 Elsevier B.V. All rights reserved.

引用

页码：57 / 62

页数：6

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