P-glycoprotein is localized in caveolae in resistant cells and in brain capillaries

被引：136

作者：

Demeule, M

Jodoin, J

Gingras, D

Béliveau, R

机构：

[1] Univ Quebec, Dept Chim Biochim, Ctr Cancerol Charles Bruneau, Mol Med Lab, Montreal, PQ H3C 3P8, Canada

[2] Univ Montreal, Grp Rech Transport Membranaire, Montreal, PQ H3C 3J7, Canada

来源：

FEBS LETTERS | 2000年 / 466卷 / 2-3期

基金：

加拿大自然科学与工程研究理事会;

关键词：

caveola; caveolin; P-glycoprotein; cyclosporin A; multidrug resistance; brain capillary;

D O I：

10.1016/S0014-5793(00)01087-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A significant proportion of P-glycoprotein (P-gp) and caveolin was co-localized in caveolae isolated from resistant (CH(R)C5) cells overexpressing P-gp and from drug-sensitive Chinese hamster ovary cells (AuxB1), The proportion of P-gp and caveolin associated with caveolar microdomains was higher in CH(R)C5 cells grown in the presence of P-gp substrates (cyclosporin A or colchicine) than in untreated CH(R)C5 cells. Coimmunoprecipitation of P-gp and caveolin from CH(R)C5 lysates suggests that there is a physical interaction between them. Furthermore, co-localization of P-gp and caveolin was found in caveolae from brain capillaries, indicating that this association also takes place in vivo. (C) 2000 Federation of European Biochemical Societies.

引用

页码：219 / 224

页数：6

共 43 条

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