Function of coenzyme F-420-dependent NADP reductase in methanogenic archaea containing an NADP-dependent alcohol dehydrogenase

被引:40
作者
Berk, H
Thauer, RK
机构
[1] MAX PLANCK INST TERR MIKROBIOL,BIOCHEM ABT,D-35043 MARBURG,GERMANY
[2] UNIV MARBURG,FACHBEREICH BIOL,MIKROBIOL LAB,D-35043 MARBURG,GERMANY
关键词
coenzyme F-420; F-420-dependent NADP reductase; F-420-dependent N-5; N-10-methylenetetrahydromethanopterin dehydrogenase; N-10-methylenetetrahydromethanopterin reductase; Methanogenic archaea; Methanogenium organophilum; Methanobacterium palustre; Methanogenium liminatans; Methanoculleus thermophilicus;
D O I
10.1007/s002030050514
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Methanogenic archaea growing on ethanol or isopropanol as the electron donor for CO2 reduction to CH4 contain either an NADP-dependent or a coenzyme F-420-dependent alcohol dehydrogenase. We report here that in both groups of methanogens, the N-5,N-10-methylenetetrahydromethanopterin dehydrogenase and the N-5,N-10-methylenetetrahydromethanopterin reductase, two enzymes involved in CO2 reduction to CH4, are specific for F-420. This raised the question how F420H2 is regenerated in the methanogens with an NADP-dependent alcohol dehydrogenase. We found that these organisms contain catabolic activities of an enzyme catalyzing the reduction of F-420 with NADPH. The F-420-dependent NADP reductase from Methanogenium organophilum was purified and characterized. The N-terminal amino acid sequence showed 42% sequence identity to a putative gene product in Methanococcus jannaschii, the total genome of which has recently been sequenced.
引用
收藏
页码:396 / 402
页数:7
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